Desulfoglucosinolate sulfotransferase
| Desulfoglucosinolate sulfotransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.8.2.24 | ||||||||
| CAS no. | 121479-85-4 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Desulfoglucosinolate sulfotransferase (EC 2.8.2.24) is an enzyme that catalyzes related chemical reactions which add a sulfate group in the final step of the biosynthesis of tryptophan- and phenylalanine-derived glucosinolates:
It uses the cofactor 3'-phosphoadenosine-5'-phosphosulfate (PAPS) and gives adenosine 3',5'-bisphosphate as a byproduct. For example, the enzyme characterised from Brassica converts desulfoglucotropaeolin to glucotropaeolin:[1]
-desulfoglucotropeolin.svg.png)
Related enzymes act to give other glucosinolates.[2][3][4]
This enzyme is a transferase, specifically a sulfotransferase, which transfer sulfur-containing groups. The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:desulfoglucosinolate sulfotransferase. Other names in common use include PAPS-desulfoglucosinolate sulfotransferase, 3'-phosphoadenosine-5'-phosphosulfate:desulfoglucosinolate, and sulfotransferase.[5]
References
- ↑ Jain, J.C.; Reed, D.W.; Grootwassink, J.W.D.; Underhill, E.W. (1989). "A radioassay of enzymes catalyzing the glucosylation and sulfation steps of glucosinolate biosynthesis in Brassica species". Analytical Biochemistry. 178 (1): 137–140. doi:10.1016/0003-2697(89)90369-2. PMID 2524977.
- ↑ Klein, Marion; Reichelt, Michael; Gershenzon, Jonathan; Papenbrock, Jutta (2006). "The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed". The FEBS Journal. 273 (1): 122–136. doi:10.1111/j.1742-4658.2005.05048.x. PMID 16367753.
- ↑ Klein, Marion; Papenbrock, Jutta (2009). "Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana". Physiologia Plantarum. 135 (2): 140–149. doi:10.1111/j.1399-3054.2008.01182.x. PMID 19077143.
- ↑ Blažević, Ivica; Montaut, Sabine; Burčul, Franko; Olsen, Carl Erik; Burow, Meike; Rollin, Patrick; Agerbirk, Niels (2020). "Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants". Phytochemistry. 169 112100. doi:10.1016/j.phytochem.2019.112100. PMID 31771793. S2CID 208318505.
- ↑ Enzyme 2.8.2.24 at KEGG Pathway Database.