+ reduced
acceptor
Ecdysone 20-monooxygenase
| ecdysone 20-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.99.22 | ||||||||
| CAS no. | 55071-97-1 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Ecdysone 20-monooxygenase (EC 1.14.99.22) is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are ecdysone, a reduced electron acceptor, and oxygen. Its products are the insect molting hormone, 20-hydroxyecdysone, the oxidised acceptor, and water.[1][2][3]
This enzyme contains cytochrome P450 acting as an oxidoreductase. The systematic name of this enzyme class is ecdysone,hydrogen-donor:oxygen oxidoreductase (20-hydroxylating). The genes in the ecdysone synthesis pathway are called halloween genes: this has the nickname shade; other names in common use include alpha-ecdysone C-20 hydroxylase, and ecdysone 20-hydroxylase.[4]
References
- ↑ Johnson P, Rees HH (1977). "The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria". Biochemical Journal. 168 (3): 513–520. doi:10.1042/bj1680513. PMC 1183799. PMID 606249.
- ↑ Nigg HN, Svoboda JA, Thompson MJ, Dutky SR, Kaplanis JN, Robbins WE (1976). "Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.)". Experientia. 32 (4): 438–439. doi:10.1007/BF01920781. PMID 5286. S2CID 8962617.
- ↑ Gilbert LI; Bollenbacher, WE; Cooper, DY; Schleyer, H; Wielgus, JJ; Gilbert, LI (1979). "Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase". Molecular and Cellular Endocrinology. 15 (3): 111–133. doi:10.1016/0303-7207(79)90033-9. PMID 488526. S2CID 25731761.
- ↑ Petryk A, Warren JT, Marques G, Jarcho MP, Gilbert LI, Kahler J, Parvy JP, Li YT, Dauphin-Villemant C, O'Connor MB (2003). "Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone". Proceedings of the National Academy of Sciences of the United States of America. 100 (24): 13773–13778. Bibcode:2003PNAS..10013773P. doi:10.1073/pnas.2336088100. PMC 283497. PMID 14610274.