3-hydroxyphenylacetate 6-hydroxylase
| 3-hydroxyphenylacetate 6-hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.13.63 | ||||||||
| CAS no. | 114705-01-0 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
3-hydroxyphenylacetate 6-hydroxylase (EC 1.14.13.63) is an enzyme that catalyzes the chemical reaction
The four substrates of this enzyme are 3-hydroxyphenylacetic acid, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are homogentisic acid, oxidised NAD+, and water. Nicotinamide adenine dinucleotide phosphate can be used as an alternative cofactor.[1][2]
This enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is 3-hydroxyphenylacetate,NAD(P)H:oxygen oxidoreductase (6-hydroxylating). This enzyme is also called 3-hydroxyphenylacetate 6-monooxygenase. It participates in styrene degradation.[1]
References
- 1 2 Enzyme 1.14.13.63 at KEGG Pathway Database.
- ↑ Van Berkel WJ, Van Den Tweel WJ (1991). "Purification and characterisation of 3-hydroxyphenylacetate 6-hydroxylase: a novel FAD-dependent monooxygenase from a Flavobacterium species". Eur. J. Biochem. 201 (3): 585–92. doi:10.1111/j.1432-1033.1991.tb16318.x. PMID 1935954.