Glyceollin synthase
| Glyceollin synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.14.135 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Glyceollin synthase is an enzyme that catalyzes the last committed step in glyceollin biosynthesis. It has been classified as a cytochrome dependent monooxygenase. It cyclizes a prenyl residue in its starting materials to convert glyceollidins (I and II) into glyceollins (I - III).[1]
This enzyme catalyzes three chemical reactions, for example:[2][3]
Glyceocarpin can alternatively be converted to glyceollin III
Glyceollin synthase is a cytochrome P450 protein containing heme. It requires a partner cytochrome P450 reductase for functional expression. This uses nicotinamide adenine dinucleotide phosphate. The systematic name of this enzyme class is 2-(or 4-)dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan,NADPH:oxygen oxidoreductase (cyclizing).[4]
The previous enzyme in the pathway to glyceollins, trihydroxypterocarpan dimethylallyltransferase also produces the isomeric compound 4-dimethylallylglycinol. This is also a substrate for glyceollin synthase:[1]
References
- 1 2 Welle R, Grisebach H (May 1988). "Induction of phytoalexin synthesis in soybean: enzymatic cyclization of prenylated pterocarpans to glyceollin isomers". Archives of Biochemistry and Biophysics. 263 (1): 191–198. doi:10.1016/0003-9861(88)90627-3. PMID 3369863.
- ↑ Bauters L, Stojilković B, Gheysen G (November 2021). "Pathogens pulling the strings: Effectors manipulating salicylic acid and phenylpropanoid biosynthesis in plants". Molecular Plant Pathology. 22 (11). British Society for Plant Pathology (W-B): 1436–1448. doi:10.1111/mpp.13123. PMC 8518561. PMID 34414650.
- ↑ Khatri P, Kuflu K, McDowell T, Lin J, Kovinich N, Dhaubhadel S (May 2025). "Discovery of three cytochrome P450 monooxygenase prenyl cyclases that catalyze the final step of glyceollin biosynthesis in soybean". Molecular Plant. 18 (5): 721–724. doi:10.1016/j.molp.2025.01.022. PMID 39891385.
- ↑ Enzyme 1.14.14.135 at KEGG Pathway Database.