+ electron
acceptor
Cytokinin dehydrogenase
| Cytokinin dehydrogenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.5.99.12 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Cytokinin dehydrogenase (EC 1.5.99.12) is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are a cytokinin such as N6-isopentenyladenine, an electron acceptor, and water. Its products are adenine, 3-methyl-2-butenal (or other aldehyde in case of different substrates), and the corresponding reduced acceptor.[1][2][3]
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with other acceptors. The systematic name of this enzyme class is N6-dimethylallyladenine:acceptor oxidoreductase. Other names in common use include N6-dimethylallyladenine:(acceptor) oxidoreductase, 6-N-dimethylallyladenine:acceptor oxidoreductase, and cytokinin oxidase/dehydrogenase abbreviated as CKX.
Structural studies
As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1W1O, PDB: 1W1Q, PDB: 1W1R, PDB: 1W1S, PDB: 2EXR, and PDB: 2Q4W. As of 2026, there have been 18 structures recorded.[4]
References
- ↑ Enzyme 1.5.99.12 at KEGG Pathway Database.
- ↑ Galuszka P, Frébort I, Sebela M, Sauer P, Jacobsen S, Pec P (January 2001). "Cytokinin oxidase or dehydrogenase? Mechanism of cytokinin degradation in cereals". European Journal of Biochemistry. 268 (2): 450–461. doi:10.1046/j.1432-1033.2001.01910.x. PMID 11168382.
- ↑ Ashikari M, Sakakibara H, Lin S, Yamamoto T, Takashi T, Nishimura A, et al. (July 2005). "Cytokinin oxidase regulates rice grain production". Science. 309 (5735): 741–745. doi:10.1126/science.1113373. PMID 15976269.
- ↑ "Cytokinin dehydrogenase". ebi.ac.uk. Protein Data Bank in Europe. Retrieved 2026-02-20.