Uridine phosphorylase
| Uridine phosphorylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.4.2.3 | ||||||||
| CAS no. | 9030-22-2 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Uridine phosphorylase (EC 2.4.2.3) is an enzyme that catalyzes a phosphorolysis reaction which converts the pyrimidine nucleotide, uridine, into uracil, by cleavage of the α-D-ribose 1-phosphate sugar unit:
This is part of a salvage pathway that allows the organisms in which the enzyme occurs to recycle the nucleotide into its component parts.[1][2][3] The enzyme has been characterised in Escherichia coli and humans.[4][5]
This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase. Other names in common use include pyrimidine phosphorylase, UrdPase, UPH, and UPase.[6]
Structural studies
As of late 2007, 27 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1K3F, PDB: 1LX7, PDB: 1RXC, PDB: 1RXS, PDB: 1RXU, PDB: 1RXY, PDB: 1RYZ, PDB: 1SJ9, PDB: 1SQ6, PDB: 1T0U, PDB: 1TGV, PDB: 1TGY, PDB: 1U1C, PDB: 1U1D, PDB: 1U1E, PDB: 1U1F, PDB: 1U1G, PDB: 1Y1Q, PDB: 1Y1R, PDB: 1Y1S, PDB: 1Y1T, PDB: 1ZL2, PDB: 2HN9, PDB: 2HRD, PDB: 2HSW, PDB: 2HWU, and PDB: 2I8A.
References
- ↑ Canellakis ES (1957). "Pyrimidine Metabolism". Journal of Biological Chemistry. 227: 329–338. doi:10.1016/S0021-9258(18)70818-1.
- ↑ Paege LM, Schlenk F (September 1952). "Bacterial uracil riboside phosphorylase". Archives of Biochemistry and Biophysics. 40 (1): 42–49. doi:10.1016/0003-9861(52)90071-4. PMID 12997187.
- ↑ Pontis H, Degerstedt G, Reichard P (July 1961). "Uridine and deoxyuridine phosphorylases from Ehrlich ascites tumor". Biochimica et Biophysica Acta. 51 (1): 138–147. doi:10.1016/0006-3002(61)91024-1. PMID 13737038.
- ↑ Leer JC, Hammer-Jespersen K, Schwartz M (May 1977). "Uridine phosphorylase from Escherichia coli. Physical and chemical characterization". European Journal of Biochemistry. 75 (1): 217–224. doi:10.1111/j.1432-1033.1977.tb11520.x. PMID 16751.
- ↑ Watanabe SI, Uchida T (November 1995). "Cloning and expression of human uridine phosphorylase". Biochemical and Biophysical Research Communications. 216 (1): 265–272. doi:10.1006/bbrc.1995.2619. PMID 7488099.
- ↑ Enzyme 2.4.2.3 at KEGG Pathway Database.