+ reduced
acceptor
Tetrachloroethene reductive dehalogenase
| Trachloroethene reductive dehalogenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.21.99.5 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Tetrachloroethene reductive dehalogenase (EC 1.21.99.5, previously 1.97.1.8) is an enzyme that catalyzes some chemical reactions which allow bacteria to metabolise chlorinated hydrocarbons.[1][2][3] For example, Dehalobacter restrictus converts tetrachloroethylene to trichloroethylene:[4][5]
The enzyme can also convert trichloroethylene into dichloroethylene and requires an electron acceptor, which is believed to be menaquinone.[6]
This enzyme is an oxidoreductase with systematic name acceptor:trichloroethene oxidoreductase (chlorinating). This enzyme is also called tetrachloroethene reductase.[7] It is one member of a family of enzymes including trichloroethene dehalogenase and vinyl chloride dehalogenase. Reductive dehalogenases are key enzymes for anaerobic respiratory process, termed organohalide respiration, and hence can be used for bioremediation.[8]
References
- ↑ Jugder BE, Ertan H, Lee M, Manefield M, Marquis CP (October 2015). "Reductive Dehalogenases Come of Age in Biological Destruction of Organohalides". Trends in Biotechnology. 33 (10): 595–610. doi:10.1016/j.tibtech.2015.07.004. PMID 26409778.
- ↑ Holliger C, Wohlfarth G, Diekert G (1998). "Reductive dechlorination in the energy metabolism of anaerobic bacteria" (PDF). FEMS Microbiol. Rev. 22 (5): 383–398. doi:10.1111/j.1574-6976.1998.tb00377.x.
- ↑ Glod G, Angst W, Holliger C, Schwarzenbach RP (1997). "Corrinoid-mediated reduction of tetrachloroethene, trichloroethene, and trichlorofluoroethene in homogeneous aqueous solution: Reaction kinetics and reaction mechanisms". Environ. Sci. Technol. 31 (1): 253–260. Bibcode:1997EnST...31..253G. doi:10.1021/es9603867.
- ↑ Schumacher W, Holliger C, Zehnder AJ, Hagen WR (June 1997). "Redox chemistry of cobalamin and iron-sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus". FEBS Letters. 409 (3): 421–5. Bibcode:1997FEBSL.409..421S. doi:10.1016/S0014-5793(97)00520-6. PMID 9224702.
- ↑ Neumann A, Wohlfarth G, Diekert G (July 1996). "Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans". The Journal of Biological Chemistry. 271 (28): 16515–9. doi:10.1074/jbc.271.28.16515. PMID 8663199.
- ↑ Schumacher W, Holliger C (April 1996). "The proton/electron ration of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus"". Journal of Bacteriology. 178 (8): 2328–33. doi:10.1128/jb.178.8.2328-2333.1996. PMC 177941. PMID 8636034.
- ↑ Enzyme 1.21.99.5 at KEGG Pathway Database.
- ↑ Jugder BE, Ertan H, Bohl S, Lee M, Marquis CP, Manefield M (2016). "Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation". Frontiers in Microbiology. 7: 249. doi:10.3389/fmicb.2016.00249. PMC 4771760. PMID 26973626.
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