Queuine tRNA-ribosyltransferase
| queuine tRNA-ribosyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.4.2.29 | ||||||||
| CAS no. | 72162-89-1 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a queuine tRNA-ribosyltransferase (EC 2.4.2.29) is an enzyme that catalyzes the chemical reaction
- [tRNA]-guanine + queuine [tRNA]-queuine + guanine
Thus, the two substrates of this enzyme are tRNA-guanine and queuine, whereas its two products are [tRNA]-queuine and guanine.
This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is [tRNA]-guanine:queuine tRNA-D-ribosyltransferase. Other names in common use include tRNA-guanine transglycosylase, guanine insertion enzyme, tRNA transglycosylase, Q-insertase, queuine transfer ribonucleate ribosyltransferase, transfer ribonucleate glycosyltransferase, tRNA guanine transglycosidase, guanine, queuine-tRNA transglycosylase, and tRNA-guanine:queuine tRNA-D-ribosyltransferase.
Structural studies
As of late 2007, 36 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1EFZ, PDB: 1ENU, PDB: 1F3E, PDB: 1IQ8, PDB: 1IT7, PDB: 1IT8, PDB: 1J2B, PDB: 1K4G, PDB: 1K4H, PDB: 1N2V, PDB: 1OZM, PDB: 1OZQ, PDB: 1P0B, PDB: 1P0D, PDB: 1P0E, PDB: 1PUD, PDB: 1PXG, PDB: 1Q2R, PDB: 1Q2S, PDB: 1Q4W, PDB: 1Q63, PDB: 1Q65, PDB: 1Q66, PDB: 1R5Y, PDB: 1S38, PDB: 1S39, PDB: 1WKD, PDB: 1WKE, PDB: 1WKF, PDB: 1Y5V, PDB: 1Y5W, PDB: 1Y5X, PDB: 2ASH, PDB: 2BBF, PDB: 2QII, and PDB: 2QZR.
References
- Howes NK, Farkas WR (1978). "Studies with a homogeneous enzyme from rabbit erythrocytes catalyzing the insertion of guanine into tRNA". J. Biol. Chem. 253 (24): 9082–7. doi:10.1016/S0021-9258(17)34288-6. PMID 721832.
- Nishimura S; Noguchi, S; Kasai, H; Shindo-Okada, N; Ohgi, T; Goto, T; Nishimura, S (1979). "Novel mechanism of post-transcriptional modification of tRNA Insertion of bases of Q precursors into tRNA by a specific tRNA transglycosylase reaction". J. Biol. Chem. 254 (8): 3067–73. Bibcode:1979JBiCh.254.3067O. doi:10.1016/S0021-9258(17)30183-7. PMID 372186.
- Shindo-Okada N, Okada N, Ohgi T, Goto T, Nishimura S (1980). "Transfer ribonucleic acid guanine transglycosylase isolated from rat liver". Biochemistry. 19 (2): 395–400. doi:10.1021/bi00543a023. PMID 6986171.