Pseudouridylate synthase
| pseudouridylate synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 4.2.1.70 | ||||||||
| CAS no. | 9023-35-2 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme pseudouridylate synthase (EC 4.2.1.70) catalyzes the chemical reaction
- uracil + D-ribose 5-phosphate pseudouridine 5′-phosphate + H2O
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is uracil hydro-lyase (adding D-ribose 5-phosphate pseudouridine-5′-phosphate-forming). Other names in common use include pseudouridylic acid synthetase, pseudouridine monophosphate synthetase, 5-ribosyluracil 5-phosphate synthetase, pseudouridylate synthetase, upsilonUMP synthetase, and uracil hydro-lyase (adding D-ribose 5-phosphate). This enzyme participates in pyrimidine metabolism.
Structural studies
As of late 2007, 22 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1DJ0, PDB: 1K8W, PDB: 1KSK, PDB: 1KSL, PDB: 1KSV, PDB: 1PRZ, PDB: 1QYU, PDB: 1R3E, PDB: 1R3F, PDB: 1SB7, PDB: 1SGV, PDB: 1SI7, PDB: 1SZW, PDB: 1V9F, PDB: 1V9K, PDB: 1VIO, PDB: 1XPI, PDB: 1Z2Z, PDB: 1ZE1, PDB: 1ZE2, PDB: 1ZL3, and PDB: 2I82.
References
- HEINRIKSON RL, GOLDWASSER E (1964). "Studies on the Biosynthesis of 5-Ribosyluracil 5'-Monophosphate in Tetrahymena Pyriformis". J. Biol. Chem. 239 (4): 1177–87. doi:10.1016/S0021-9258(18)91409-2. PMID 14165924.
- Matsushita T, Davis FF (1971). "Studies on pseudouridylic acid synthetase from various sources". Biochim. Biophys. Acta. 238 (2): 165–73. doi:10.1016/0005-2787(71)90082-7. PMID 4936431.
- Remsen JF, Matsushita T, Chirikjian JG, Davis FF (1972). "Enzymatic synthesis of deoxypseudouridylic acid and a study of certain of its properties". Biochim. Biophys. Acta. 281 (4): 481–7. doi:10.1016/0005-2787(72)90148-7. PMID 4569284.
- Suzuki T, Hochster RM (1966). "On the biosynthesis of pseudouridine and of pseudouridylic acid in Agrobacterium tumefaciens". Can. J. Biochem. 44 (2): 259–72. doi:10.1139/o66-029. PMID 5942965.