Hyaluronate lyase
| hyaluronate lyase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 4.2.2.1 | ||||||||
| CAS no. | 37259-53-3 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme hyaluronate lyase (EC 4.2.2.1) catalyzes the chemical reaction
- Cleaves hyaluronate chains at a β-D-GalNAc-(1→4)-β-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is hyaluronate lyase. Other names in common use include hyaluronidase (ambiguous), (hyalurononglucosaminidase) (ambiguous), (hyaluronoglucuronidase)], glucuronoglycosaminoglycan lyase, spreading factor, and mucinase (ambiguous).
Structural studies
As of late 2007, 27 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1C82, PDB: 1EGU, PDB: 1F1S, PDB: 1F9G, PDB: 1I8Q, PDB: 1LOH, PDB: 1LXK, PDB: 1LXM, PDB: 1N7N, PDB: 1N7O, PDB: 1N7P, PDB: 1N7Q, PDB: 1N7R, PDB: 1OJM, PDB: 1OJN, PDB: 1OJO, PDB: 1OJP, PDB: 1W3Y, PDB: 2BRP, PDB: 2BRV, PDB: 2BRW, PDB: 2C3F, PDB: 2DP5, PDB: 2PK1, PDB: 2YVV, PDB: 2YW0, and PDB: 2YX2.
References
- Linker A, Hoffman P, Meyer K, Sampson P, Korn ED (1960). "The formation of unsaturated disaccharides from mucopoly-saccharides and their cleavage to alpha-keto acid by bacterial enzymes". Journal of Biological Chemistry. 235 (11): 3061–5. doi:10.1016/S0021-9258(20)81309-X. PMID 13762462.
- Meyer K, Rapport MM (1952). "Hyaluronidases". Advances in Enzymology and Related Areas of Molecular Biology. Advances in Enzymology - and Related Areas of Molecular Biology. Vol. 13. pp. 199–236. doi:10.1002/9780470122587.ch6. ISBN 978-0-470-64614-4. PMID 14943668.
{{cite book}}: ISBN / Date incompatibility (help) - Moran F, Nasuno S, Starr MP (1968). "Extracellular and intracellular polygalalacturonic acid trans-eliminases of Erwinia carotovora". Archives of Biochemistry and Biophysics. 123 (2): 298–306. Bibcode:1968ArBB..123..298M. doi:10.1016/0003-9861(68)90138-0. PMID 5642600.