Phosphomethylpyrimidine kinase
| Phosphomethylpyrimidine kinase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 | |||||||||
| Identifiers | |||||||||
| EC no. | 2.7.4.7 | ||||||||
| CAS no. | 37278-18-5 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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Phosphomethylpyrimidine kinase (EC 2.7.4.7) is an enzyme that catalyzes the chemical reaction
The enzyme characterised from baker's yeast converts 4-amino-2-methyl-5-phosphooxymethylpyrimidine to 4-amino-2-methyl-5-diphosphooxymethylpyrimidine by transferring a phosphate group from the cofactor, adenosine triphosphate (ATP), which is converted to adenosine diphosphate (ADP). The starting material is biosynthesized from toxopyrimidine by the enzyme hydroxymethylpyrimidine kinase.[1] These reactions are steps in the biosynthesis of thiamine (vitamin B1).[2]
This enzyme is a transferase, specifically one transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor. The systematic name of this enzyme class is ATP:(4-amino-2-methylpyrimidin-5-yl)methyl-phosphate phosphotransferase. Other names in common use include hydroxymethylpyrimidine phosphokinase, and ATP:4-amino-2-methyl-5-phosphomethylpyrimidine phosphotransferase.[3]
Structural studies
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1JXH, PDB: 1JXI, PDB: 1UB0, and PDB: 2I5B.
References
- ↑ Lewin LM, Brown GM (1961). "The biosynthesis of thiamine. III. Mechanism of enzymatic formation of the pyrophosphate ester of 2-methyl-4-amino-5-hydroxymethylpyrimidine". J. Biol. Chem. 236 (10): 2768–2771. doi:10.1016/S0021-9258(19)61734-5.
- ↑ Begley TP (February 2006). "Cofactor biosynthesis: an organic chemist's treasure trove". Natural Product Reports. 23 (1): 15–25. doi:10.1039/b207131m. PMID 16453030.
- ↑ Enzyme 2.7.4.7 at KEGG Pathway Database.