Leucoanthocyanidin reductase
| Leucoanthocyanidin reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.17.1.3 | ||||||||
| CAS no. | 93389-48-1 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Leucoanthocyanidin reductase (EC 1.17.1.3) (LAR, aka leucocyanidin reductase or LCR) is an enzyme that catalyzes several related chemical reduction steps in flavonoid biosynthesis. For example, it converts leucocyanidin to catechin.[1]
-Catechin.svg.png)
The three substrates of this enzyme are leucocyanidin, reduced nicotinamide adenine dinucleotide phosphate (NADPH), and a proton. Its products are catechin, oxidised NADP+, and water.[2][3]
This enzyme is an oxidoreductase, with the systematic name (2R,3S)-catechin:NADP+ 4-oxidoreductase. It is also called leucocyanidin reductase.
Other reactions catalysed
The enzyme also converts leucopelargonidin to afzelechin and leucodelphinidin to gallocatechol:[1][2]
-Gallocatechin.svg.png)
It can be found in the plant Hedysarum sulphurescens and in Vitis vinifera (grape).[4]
References
- 1 2 Tanner GJ, Francki KT, Abrahams S, Watson JM, Larkin PJ, Ashton AR (August 2003). "Proanthocyanidin biosynthesis in plants. Purification of legume leucoanthocyanidin reductase and molecular cloning of its cDNA". The Journal of Biological Chemistry. 278 (34): 31647–31656. doi:10.1074/jbc.M302783200. PMID 12788945.
- 1 2 Enzyme 1.17.1.3 at KEGG Pathway Database.
- ↑ Tanner GJ, Kristiansen KN (March 1993). "Synthesis of 3,4-cis-[3H]leucocyanidin and enzymatic reduction to catechin". Analytical Biochemistry. 209 (2): 274–277. doi:10.1006/abio.1993.1119. PMID 8470799.
- ↑ Maugé C, Granier T, d'Estaintot BL, Gargouri M, Manigand C, Schmitter JM, et al. (April 2010). "Crystal structure and catalytic mechanism of leucoanthocyanidin reductase from Vitis vinifera". Journal of Molecular Biology. 397 (4): 1079–1091. doi:10.1016/j.jmb.2010.02.002. PMID 20138891.