Glucosyl-3-phosphoglycerate synthase

Glucosyl-3-phosphoglycerate synthase
Identifiers
EC no.2.4.1.266
Databases
BRENDAenzyme data
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Glucosyl-3-phosphoglycerate synthase (EC 2.4.1.266, GpgS protein, GPG synthase, glucosylphosphoglycerate synthase) is an enzyme with systematic name NDP-glucose:3-phospho-D-glycerate 2-alpha-D-glucosyltransferase.[1][2] It catalyses the following chemical reaction

2D representation of the chemical structure of Q223118.
3-phospho-D-glyceric acid
+ UDP-glucose
 
 
 
Rightward reaction arrow with minor product(s) to top right
 
 
 
2D representation of the chemical structure of Q27132035.
2-O-(α-D-glucopyranosyl)-3-O-phospho-D-glyceric acid

This transfers a glucose unit to 3-phospho-D-glyceric acid to give its glucosyl derivative. The enzyme characterised from Persephonella marina can use GDP-glucose or ADP-glucose in the reaction.[3] However, the enzyme in Methanococcoides burtonii can only use GDP-glucose.[4] The enzyme is also present in Mycobacterium tuberculosis.[5][6] In these organisms, a second reaction catalysed by glucosyl-3-phosphoglycerate phosphatase forms 2-O-(α-D-glucopyranosyl)-D-glyceric acid by removal of the phosphate group.[7]

References

  1. ↑ Empadinhas N, Albuquerque L, Mendes V, Macedo-Ribeiro S, da Costa MS (March 2008). "Identification of the mycobacterial glucosyl-3-phosphoglycerate synthase". FEMS Microbiology Letters. 280 (2): 195–202. doi:10.1111/j.1574-6968.2007.01064.x. PMID 18221489.
  2. ↑ Kaur D, Pham H, Larrouy-Maumus G, Rivière M, Vissa V, Guerin ME, Puzo G, Brennan PJ, Jackson M (2009). Herman C (ed.). "Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria". PLOS ONE. 4 (5) e5447. Bibcode:2009PLoSO...4.5447K. doi:10.1371/journal.pone.0005447. PMC 2674218. PMID 19421329.
  3. ↑ Costa J, Empadinhas N, da Costa MS (March 2007). "Glucosylglycerate biosynthesis in the deepest lineage of the Bacteria: characterization of the thermophilic proteins GpgS and GpgP from Persephonella marina". Journal of Bacteriology. 189 (5): 1648–54. doi:10.1128/JB.00841-06. PMC 1855766. PMID 17189358.
  4. ↑ Costa J, Empadinhas N, Gonçalves L, Lamosa P, Santos H, da Costa MS (February 2006). "Characterization of the biosynthetic pathway of glucosylglycerate in the archaeon Methanococcoides burtonii". Journal of Bacteriology. 188 (3): 1022–30. doi:10.1128/JB.188.3.1022-1030.2006. PMC 1347341. PMID 16428406.
  5. ↑ Gest P, Kaur D, Pham HT, van der Woerd M, Hansen E, Brennan PJ, Jackson M, Guerin ME (December 2008). "Preliminary crystallographic analysis of GpgS, a key glucosyltransferase involved in methylglucose lipopolysaccharide biosynthesis in Mycobacterium tuberculosis". Acta Crystallographica Section F. 64 (Pt 12): 1121–4. doi:10.1107/S1744309108032892. PMC 2593697. PMID 19052364.
  6. ↑ Pereira PJ, Empadinhas N, Albuquerque L, Sá-Moura B, da Costa MS, Macedo-Ribeiro S (2008). Song H (ed.). "Mycobacterium tuberculosis glucosyl-3-phosphoglycerate synthase: structure of a key enzyme in methylglucose lipopolysaccharide biosynthesis". PLOS ONE. 3 (11) e3748. Bibcode:2008PLoSO...3.3748P. doi:10.1371/journal.pone.0003748. PMC 2581804. PMID 19015727.
  7. ↑ Enzyme 2.4.1.266 at KEGG Pathway Database.
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