+ reduced
acceptor
Cholesterol 25-hydroxylase
| Cholesterol 25-hydroxylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.99.38 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Cholesterol 25-hydroxylase (EC 1.14.99.38) is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are cholesterol, a reduced electron acceptor, and oxygen. Its products are 25-hydroxycholesterol, the electron acceptor, and water.[1][2]
This enzyme is an oxidoreductase, that uses molecular oxygen as oxidant and contains a di-iron (Fe-O-Fe) cofactor.[3] The systematic name of this enzyme class is cholesterol, hydrogen-donor:oxygen oxidoreductase (25-hydroxylating). It is also called cholesterol 25-monooxygenase.[4]
Transcripts for this enzyme have been identified in macrophages from the testis.[5][6]
References
- ↑ Lund EG, Kerr TA, Sakai J, Li WP, Russell DW (December 1998). "cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism". The Journal of Biological Chemistry. 273 (51): 34316–27. doi:10.1074/jbc.273.51.34316. PMID 9852097.
- ↑ Russell DW (2003). "The enzymes, regulation, and genetics of bile acid synthesis". Annual Review of Biochemistry. 72: 137–74. doi:10.1146/annurev.biochem.72.121801.161712. PMID 12543708.
- ↑ Fox BG, Shanklin J, Ai J, Loehr TM, Sanders-Loehr J (November 1994). "Resonance Raman evidence for an Fe-O-Fe center in stearoyl-ACP desaturase. Primary sequence identity with other diiron-oxo proteins". Biochemistry. 33 (43): 12776–86. doi:10.1021/bi00209a008. PMID 7947683.
- ↑ Enzyme 1.14.99.38 at KEGG Pathway Database.
- ↑ Chen JJ, Lukyanenko Y, Hutson JC (May 2002). "25-hydroxycholesterol is produced by testicular macrophages during the early postnatal period and influences differentiation of Leydig cells in vitro". Biology of Reproduction. 66 (5): 1336–41. doi:10.1095/biolreprod66.5.1336. PMID 11967195. S2CID 22637279.
- ↑ Lukyanenko Y, Chen JJ, Hutson JC (November 2002). "Testosterone regulates 25-hydroxycholesterol production in testicular macrophages". Biology of Reproduction. 67 (5): 1435–8. doi:10.1095/biolreprod.102.007575. PMID 12390873.