4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
| 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.7.1.148 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (EC 2.7.1.148) is an enzyme that catalyzes the chemical reaction
The enzyme characterised from Escherichia coli converts 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) to its 2-phosphate derivative, 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-MEP) by transferring a phosphate group from the cofactor, adenosine triphosphate (ATP), which is converted to adenosine diphosphate (ADP).[1] The reaction is part of the non-mevalonate pathway to terpenes.[2]
This enzyme is a transferase, specifically one transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol 2-phosphotransferase. This enzyme is also called CDP-ME kinase, and IspE.[3]
Structural studies
As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1OJ4, PDB: 1UEK, PDB: 2V2Q, PDB: 2V2V, PDB: 2V2Z, PDB: 2V34, and PDB: 2V8P.
References
- ↑ Lüttgen H, Rohdich F, Herz S, Wungsintaweekul J, Hecht S, Schuhr CA, Fellermeier M, Sagner S, Zenk MH, Bacher A, Eisenreich W (2000). "Biosynthesis of terpenoids: YchB protein of Escherichia coli phosphorylates the 2-hydroxy group of 4-diphosphocytidyl-2C-methyl-d-erythritol". Proc. Natl. Acad. Sci. U.S.A. 97 (3): 1062–7. Bibcode:2000PNAS...97.1062L. doi:10.1073/pnas.97.3.1062. PMC 15522. PMID 10655484.
- ↑ Kuzuyama T, Takagi M, Kaneda K, Watanabe H, Dairi T, Seto H (2000). "Studies on the nonmevalonate pathway: conversion of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol to its 2-phospho derivative by 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase". Tetrahedron Lett. 41 (16): 2925–2928. doi:10.1016/S0040-4039(00)00295-1.
- ↑ Enzyme 2.7.1.148 at KEGG Pathway Database.
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