Aristolochene synthase
| aristolochene synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 4.2.3.9 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme aristolochene synthase (EC 4.2.3.9) catalyzes the chemical reaction
This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is (2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, aristolochene-forming). Other names in common use include sesquiterpene cyclase, trans, trans-farnesyl diphosphate aristolochene-lyase, trans, trans-farnesyl-diphosphate diphosphate-lyase (cyclizing and aristolochene-forming). This enzyme participates in terpenoid biosynthesis.
This protein may use the morpheein model of allosteric regulation.[1]
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes PDB: 2E4O and PDB: 2OA6. They are both notable for the very high helix content of the structure.
References
- ↑ T. Selwood & E. K. Jaffe (2011). "Dynamic dissociating homo-oligomers and the control of protein function". Arch. Biochem. Biophys. 519 (2): 131–43. doi:10.1016/j.abb.2011.11.020. PMC 3298769. PMID 22182754.
Further reading
- Cane DE, Prabhakaran PC, Oliver JS, McIlwaine DB (1990). "Aristolochene biosynthesis. Stereochemistry of the deprotonation steps in the enzymatic cyclization of farnesyl pyrophosphate". J. Am. Chem. Soc. 112 (8): 3209–3210. Bibcode:1990JAChS.112.3209C. doi:10.1021/ja00164a051.
- Noguchi H, Rawlings BJ (1989). "Aristolochene biosynthesis and enzymatic cyclization of farnesyl pyrophosphate". J. Am. Chem. Soc. 111 (24): 8914–8916. Bibcode:1989JAChS.111.8914C. doi:10.1021/ja00206a022.
- Hohn TM, Plattner RD (1989). "Purification and characterization of the sesquiterpene cyclase aristolochene synthase from Penicillium roqueforti". Arch. Biochem. Biophys. 272 (1): 137–43. doi:10.1016/0003-9861(89)90204-X. PMID 2544140.
- Proctor RH, Hohn TM (1993). "Aristolochene synthase. Isolation, characterization, and bacterial expression of a sesquiterpenoid biosynthetic gene (Ari1) from Penicillium roqueforti". J. Biol. Chem. 268 (6): 4543–8. doi:10.1016/S0021-9258(18)53644-9. PMID 8440737.
- Calvert MJ, Ashton PR, Allemann RK (2002). "Germacrene A is a product of the aristolochene synthase-mediated conversion of farnesylpyrophosphate to aristolochene". J. Am. Chem. Soc. 124 (39): 11636–41. Bibcode:2002JAChS.12411636C. doi:10.1021/ja020762p. PMID 12296728.