Alpha,alpha-trehalose synthase
| Alpha,alpha-trehalose synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.4.1.245 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Alpha,alpha-trehalose synthase (EC 2.4.1.245, trehalose synthase, trehalose synthetase, UDP-glucose:glucose 1-glucosyltransferase, TreT, PhGT) is an enzyme with systematic name ADP-glucose:D-glucose 1-alpha-D-glucosyltransferase. This enzyme catalyses the following chemical reaction
This reversible reaction forms trehalose by combining D-glucose with a second glucose unit which is attached to the phosphate group of a nucleoside such as uridine diphosphate (UDP) or adenosine diphosphate.[1] The enzyme first characterised from Thermococcus litoralis requires magnesium ion, Mg2+ for maximal activity.[2] It has also been found in other thermophilic bacteria including Pyrococcus horikoshii,[3] Rubrobacter xylanophilus,[4] and Thermoproteus tenax.[5]
References
- ↑ Enzyme 2.4.1.245 at KEGG Pathway Database.
- ↑ Qu, Qiuhao; Lee, Sung-Jae; Boos, Winfried (2004). "TreT, a Novel Trehalose Glycosyltransferring Synthase of the Hyperthermophilic Archaeon Thermococcus litoralis". Journal of Biological Chemistry. 279 (46): 47890–47897. doi:10.1074/jbc.m404955200. PMID 15364950.
- ↑ Ryu, Soo-In; Park, Cheon-Seok; Cha, Jaeho; Woo, Eui-Jeon; Lee, Soo-Bok (2005). "A novel trehalose-synthesizing glycosyltransferase from Pyrococcus horikoshii: Molecular cloning and characterization". Biochemical and Biophysical Research Communications. 329 (2): 429–436. doi:10.1016/j.bbrc.2005.01.149. PMID 15737605.
- ↑ Nobre, Ana; Alarico, Susana; Fernandes, Chantal; Empadinhas, Nuno; Da Costa, Milton S. (2008). "A Unique Combination of Genetic Systems for the Synthesis of Trehalose in Rubrobacter xylanophilus : Properties of a Rare Actinobacterial TreT". Journal of Bacteriology. 190 (24): 7939–7946. doi:10.1128/JB.01055-08. PMC 2593226. PMID 18835983.
- ↑ Kouril, Theresa; Zaparty, Melanie; Marrero, Jeannette; Brinkmann, Henner; Siebers, Bettina (2008). "A novel trehalose synthesizing pathway in the hyperthermophilic Crenarchaeon Thermoproteus tenax: The unidirectional TreT pathway". Archives of Microbiology. 190 (3): 355–369. doi:10.1007/s00203-008-0377-3. PMID 18483808.
External links
- Alpha,alpha-trehalose+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
Category: