4-Nitrocatechol 4-monooxygenase

4-nitrocatechol 4-monooxygenase
Identifiers
EC no.1.14.13.166
Databases
BRENDAenzyme data
ExPASyNiceZyme view
KEGGenzyme entry
MetaCycmetabolic pathway
Rheareactions
PDB structuresRCSB PDB PDBe PDBsum
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PMCarticles
PubMedarticles
NCBIproteins

4-nitrocatechol 4-monooxygenase (EC 1.14.13.166) is an enzyme with systematic name 4-nitrocatechol,NAD(P)H:oxygen 4-oxidoreductase (4-hydroxylating, nitrite-forming).[1][2]This enzyme catalyses the following chemical reaction:

2D representation of the chemical structure of Q230010.
4-nitrocatechol
+ NADH + H+
 
 
O2
H2O
Rightward reaction arrow with minor substrate(s) from top left and minor product(s) to top right
 
 
 
2D representation of the chemical structure of hydroxy-1,4-benzoquinone .
hydroxy-1,4-benzoquinone
+ NAD+ + HNO2
 

The four substrates of this enzyme are 4-nitrocatechol, reduced nicotinamide adenine dinucleotide (NADH), oxygen, and a proton. Its products are hydroxy-1,4-benzoquinone, oxidised NAD+, water, and nitrous acid. It is a flavoprotein that uses flavin adenine dinucleotide as a cofactor.[3][4]

References

  1. ↑ Kadiyala V, Spain JC (July 1998). "A two-component monooxygenase catalyzes both the hydroxylation of p-nitrophenol and the oxidative release of nitrite from 4-nitrocatechol in Bacillus sphaericus JS905". Applied and Environmental Microbiology. 64 (7): 2479–84. Bibcode:1998ApEnM..64.2479K. doi:10.1128/AEM.64.7.2479-2484.1998. PMC 106414. PMID 9647818.
  2. ↑ Kitagawa W, Kimura N, Kamagata Y (August 2004). "A novel p-nitrophenol degradation gene cluster from a gram-positive bacterium, Rhodococcus opacus SAO101". Journal of Bacteriology. 186 (15): 4894–902. doi:10.1128/JB.186.15.4894-4902.2004. PMC 451640. PMID 15262926.
  3. ↑ Enzyme 1.14.13.166 at KEGG Pathway Database.
  4. ↑ Zhang JJ, Liu H, Xiao Y, Zhang XE, Zhou NY (April 2009). "Identification and characterization of catabolic para-nitrophenol 4-monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain WBC-3". Journal of Bacteriology. 191 (8): 2703–10. doi:10.1128/JB.01566-08. PMC 2668391. PMID 19218392.
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