Total oxyradical scavenging capacity

A total oxyradical scavenging capacity (TOSC) assay is a method of quantifying antioxidant capacity in the context of molecular biology[1] and toxicology, as an indication of oxidative stress.[2] The assay measures ethylene (ethene).[3][4]

The invention of this assay was first made public during 1998.[4]

See also

References

  1. ↑ Garrett, A.; Murray, B.; Robison, R.; O'Neill, K. (30 September 2009). "Measuring Antioxidant Capacity Using the ORAC and TOSC Assays". In Donald Armstrong (ed.). Advanced Protocols in Oxidative Stress II. Methods in Molecular Biology. Vol. 594. Totowa, NJ.: Humana Press. doi:10.1007/978-1-60761-411-1_17. ISBN 978-1-60761-410-4.
  2. ↑ Regoli, Francesco (October 2000). Willie Peijnenburg; Wen-Xiong Wang (eds.). "Total oxyradical scavenging capacity (TOSC) in polluted and translocated mussels: a predictive biomarker of oxidative stress". Aquatic Toxicology. 50 (4). Ancona, Italy: Elsevier B.V.: 351–361. Bibcode:2000AqTox..50..351R. doi:10.1016/S0166-445X(00)00091-6. ISSN 0166-445X. PMID 10967397.
  3. ↑ Winston, G; Regoli, F; Dugas Jr, A; Fong, J; Blanchard, K (1998). "A rapid gas chromatographic assay for determining oxyradical scavenging capacity of antioxidants and biological fluids". Free Radic Biol Med. 24: 480–493. PMID 9438561. Peroxyl radicals generated by thermal homolysis of 2,2'-azobis-amidinopropane (ABAP) cause the oxidation of alpha-keto-gamma-methiolbutyric acid (KMBA) to ethylene; ethylene formation is monitored by gas chromatographic analysis of head space from the reaction vessel.
  4. 1 2 Senti T. Senthilmohan; Brett M Davis; Paul F. Wilson; Murray J. McEwan (2009). "Improved peroxyl radical scavenging TOSC assay to quantify antioxidant capacity using SIFT-MS". Redox Report. 14 (5): 198. doi:10.1179/135100009X12525712409571. The TOSC assay (which is an example of a HAT assay) was introduced by Winston et al.11 in 1998

Further reading

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