S-formylglutathione hydrolase
| S-formylglutathione hydrolase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.2.12 | ||||||||
| CAS no. | 50812-21-0 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme S-formylglutathione hydrolase (EC 3.1.2.12) catalyzes the reaction
The enzyme characterised from human liver hydrolyses S-formylglutathione to glutathione and formic acid, which forms the thioester in the starting material.[1][2] The enzyme is part of a biochemical pathway that removes formaldehyde, which is toxic to many organisms.[3][4] The structure of the enzyme from Pseudoalteromonas haloplanktis has been determined by X-ray crystallography.[5]
This enzyme is a hydrolase, specifically one acting on thioester bonds. The systematic name is S-formylglutathione hydrolase.[6]
References
- ↑ Uotila L (1973). "Preparation and assay of glutathione thiol esters. Survey of human liver glutathione thiol esterases". Biochemistry. 12 (20): 3938–43. doi:10.1021/bi00744a024. PMID 4200890.
- ↑ Uotila L, Koivusalo M (1974). "Purification and properties of S-formylglutathione hydrolase from human liver". J. Biol. Chem. 249 (23): 7664–72. doi:10.1016/S0021-9258(19)81288-7. PMID 4436331.
- ↑ Harms N, Ras J, Reijnders WN, van Spanning RJ, Stouthamer AH (1996). "S-formylglutathione hydrolase of Paracoccus denitrificans is homologous to human esterase D: a universal pathway for formaldehyde detoxification?". J. Bacteriol. 178 (21): 6296–9. doi:10.1128/jb.178.21.6296-6299.1996. PMC 178503. PMID 8892832.
- ↑ Gonzalez, Claudio F.; Proudfoot, Michael; Brown, Greg; et al. (2006). "Molecular Basis of Formaldehyde Detoxification". Journal of Biological Chemistry. 281 (20): 14514–14522. doi:10.1074/jbc.M600996200. PMID 16567800.
- ↑ Alterio, Vincenzo; Aurilia, Vincenzo; Romanelli, Alessandra; et al. (2010). "Crystal structure of an S -formylglutathione hydrolase from Pseudoalteromonas haloplanktis TAC125". Biopolymers. 93 (8): 669–677. doi:10.1002/bip.21420. PMID 20209484.
- ↑ Enzyme 3.1.2.12 at KEGG Pathway Database.
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