Queuosine 5'-phosphate N-glycosylase/hydrolase (also called queuosine salvage protein) is protein which in humans is encoded by the gene QNG1 (previously C9orf64).[5] The function and biological process of the queuosine salvage protein is a queuosine-nucleotide N-glycosylase/hydrolase (QNG1) that releases queuine from Q-5'-monophosphate, and this activity is required for
the salvage of queuine from exogenous Queuosine by S. pombe and HeLa
cells.[6] Some evidence from orthologs indicates it may be involved in tRNA processing and recycling. The most common mRNA contains 4 coding exons, and it has 2 additional alternatively spliced exons.[5] QNG1 has been found in 5 different splice variants.[7]
Expression of this gene is highest in the duodenum and small intestine, and it is also expressed in 24 other tissues.[8]
Protein
Queuosine salvage protein is 341 amino acids long with a molecular weight of 39,029 daltons and an isoelectric point of 5.61. The crystal structures of wild-type human
QNG1 and QNG1 in complex with queuine have been deposited with the
Protein Data Bank under accession numbers 7UGK and 8DL3.[9]
Gene locus
QNG1 is located on chromosome 9q21.32.[5] The genes closest to QNG1 on the long arm of chromosome 9 include GKAP1, KIF27, HNRNPK, RMI1, and a MicroRNA MIR7-1.[10]
References
- 1 2 3 GRCh38: Ensembl release 89: ENSG00000165118 – Ensembl, May 2017
- 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000021550 – Ensembl, May 2017
- ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- 1 2 3 "Entrez Gene: C9orf64".
- ↑ Hung SH, Elliott GI, Ramkumar TR, Burtnyak L, McGrenaghan CJ, Alkuzweny S, Quaiyum S, Iwata-Reuyl D, Pan X, Green BD, Kelly VP, De Crécy-Lagard V, Swairjo MA (2023). "Structural basis of Qng1-mediated salvage of the micronutrient queuine from queuosine-5′-monophosphate as the biological substrate". Nucleic Acids Research. 51 (2): 935–951. doi:10.1093/nar/gkac1231. PMC 9881137. PMID 36610787.
- ↑ "NCBI: AceView".
- ↑ "C9orf64 chromosome 9 open reading frame 64 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2018-06-02.
- ↑ "PDB:8DL3".
- ↑ "Entrez Gene: C9orf64 chromosome 9 open reading frame 64 [Homo sapiens]".
Further reading
- Hung SH, Elliott GI, Ramkumar TR, Burtnyak L, McGrenaghan CJ, Alkuzweny S, Quaiyum S, Iwata-Reuyl D, Pan X, Green BD, Kelly VP, de Crécy-Lagard V, Swairjo MA (2023). "Structural basis of Qng1-mediated salvage of the micronutrient queuine from queuosine-5'-monophosphate as the biological substrate". Nucleic Acids Research. 51 (2): 935–951. doi:10.1093/nar/gkac1231. PMC 9881137. PMID 36610787.
- Cai LY, Abe M, Izumi S, Imura M, Yasugi T, Ushijima T (2007). "Identification of PRTFDC1 silencing and aberrant promoter methylation of GPR150, ITGA8 and HOXD11 in ovarian cancers". Life Sciences. 80 (16): 1458–65. doi:10.1016/j.lfs.2007.01.015. PMID 17303177.
- Sweetser DA, Peniket AJ, Haaland C, Blomberg AA, Zhang Y, Zaidi ST, Dayyani F, Zhao Z, Heerema NA, Boultwood J, Dewald GW, Paietta E, Slovak ML, Willman CL, Wainscoat JS, Bernstein ID, Daly SB (2005). "Delineation of the minimal commonly deleted segment and identification of candidate tumor-suppressor genes in del(9q) acute myeloid leukemia". Genes, Chromosomes and Cancer. 44 (3): 279–91. doi:10.1002/gcc.20236. PMID 16015647. S2CID 25536746.
