Putrescine aminotransferase
| Putrescine aminotransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.6.1.82 | ||||||||
| CAS no. | 98982-73- | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Putrescine aminotransferase (EC 2.6.1.82, putrescine-alpha-ketoglutarate transaminase, YgjG, putrescine:alpha-ketoglutarate aminotransferase, PAT, putrescine:2-oxoglutarate aminotransferase, putrescine transaminase) is an enzyme with systematic name butane-1,4-diamine:2-oxoglutarate aminotransferase.[1] This pyridoxal phosphate-dependent enzyme catalyses the following chemical reaction
The two substrates of this enzyme first characterised Escherichia coli are putrescine and α-ketoglutaric acid. Its initial products are 4-aminobutanal and L-glutamic acid.[2] In this bacterium, the reaction is part of the breakdown pathway of the amino acid arginine.[3][4]
4-aminobutanal is unstable under these reaction conditions as its aldehyde functional group can cyclise readily with the amino group, losing water:
4-aminobutanal or 1-pyrroline are the starting substrates for the next enzyme in the pathway, aminobutyraldehyde dehydrogenase.[3]
References
- ↑ Enzyme 2.6.1.82 at KEGG Pathway Database.
- ↑ Prieto-Santos MI, Martín-Checa J, Balaña-Fouce R, Garrido-Pertierra A (February 1986). "A pathway for putrescine catabolism in Escherichia coli". Biochimica et Biophysica Acta (BBA) - General Subjects. 880 (2–3): 242–4. doi:10.1016/0304-4165(86)90085-1. PMID 3510672.
- 1 2 Samsonova NN, Smirnov SV, Novikova AE, Ptitsyn LR (August 2005). "Identification of Escherichia coli K12 YdcW protein as a gamma-aminobutyraldehyde dehydrogenase". FEBS Letters. 579 (19): 4107–12. Bibcode:2005FEBSL.579.4107S. doi:10.1016/j.febslet.2005.06.038. PMID 16023116.
- ↑ Samsonova NN, Smirnov SV, Altman IB, Ptitsyn LR (January 2003). "Molecular cloning and characterization of Escherichia coli K12 ygjG gene". BMC Microbiology. 3 (1) 2. doi:10.1186/1471-2180-3-2. PMC 150594. PMID 12617754.
External links
- Putrescine+aminotransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)