Pseudoalteromonas translucida
Pseudoalteromonas translucida is a marine bacterium isolated from the Gulf of Peter the Great in the sea of Japan
Description
Pseudoalteromonas translucida is a Gram-negative, rod-shaped bacterium that measures roughly 0.7–0.9 µm in diameter.[1] It consists of single cells that move using polar flagella.[1]
The colony appearance is distinctly pale-orange-pigmented and translucent, which gives the species its name.[1] It is a strictly aerobic chemoorganotroph with a respiratory metabolism.[1] It does not form endospores, lacks an arginine dihydrolase system, and does not accumulate poly-β-hydroxybutyrate as an internal cellular reserve.[1] The bacterium is oxidase- and catalase-positive.[1]
Physiology and cultivation
P. translucida requires sodium ions (Na+) or sea water to support growth, developing optimally in environments with a 1–6% NaCl concentration.[1] The culture grows within a temperature range of 4 °C to 30 °C, with an optimal growth temperature of 25 °C; it fails to grow at 35 °C.[1]
Physiologically, it is capable of decomposing elastin and degrading gelatin, DNA, and Tween 80.[1] Biochemically, its genomic DNA G+C content is measured at 46.3 mol%.[1] A specific strain (strain TAC 125) produces a fatty acid cis/trans isomerase that alters cell membrane fluidity to survive high-stress, toxic cellular environments.[2]
Genome
The complete genome sequence of the type strain (KMM 520ᵀ) reveals a multipartite architecture spanning 4,147,593 base pairs.[3] Uniquely, its genome is split across two distinct circular chromosomes.[3] Chromosome II contains a plasmid-type replication initiator protein (RepA), suggesting it originally evolved from a historical plasmid integration event.[3]
Isolation
The type strain, KMM 520ᵀ (also cataloged as LMG 19696ᵀ or ATCC BAA-3157ᵀ), was originally isolated from marine environmental samples collected from the Gulf of Peter the Great within the Sea of Japan.[1] Samples were gathered from both seawater and marine sponge tissue hosts.[1]
References
- 1 2 3 4 5 6 7 8 9 10 11 12 Ivanova, Elena P.; Sawabe, Tomoo; Lysenko, Anatolii M.; Gorshkova, Nataliya M. (2002). "Pseudoalteromonas translucida sp. nov. and Pseudoalteromonas paragorgicola sp. nov., and emended description of the genus". International Journal of Systematic and Evolutionary Microbiology. 52 (5): 1759–1766. doi:10.1099/00207713-52-5-1759. PMID 12361284.
- ↑ Médigue, Claudine; Krin, Evelyne; Pascal, Géraldine; Barbe, Valérie; et al. (October 2005). "Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125". Genome Research. 15 (10): 1325–1335. doi:10.1101/gr.4126905. ISSN 1088-9051. PMC 1240074. PMID 16169927.
- 1 2 3 Rong, J.-C.; Liu, M.; Li, Y.; Sun, T.-Y. (2016). "Complete genome sequence of a marine bacterium with two chromosomes, Pseudoalteromonas translucida KMM 520T". Marine Genomics. 26: 17–20. doi:10.1016/j.margen.2016.03.003.