Precorrin-4 C11-methyltransferase
| Precorrin-4 C11-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.133 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Precorrin-4 C11-methyltransferase (EC 2.1.1.133) is an enzyme that catalyzes the chemical reaction
This is a methylation reaction in which the cobalamin precursor, precorrin-4, is converted to precorrin-4. The methyl group comes from the cofactor, S-adenosyl methionine (SAM), which becomes S-adenosyl-L-homocysteine (SAH). The enzyme was characterised from Pseudomonas denitrificans and Salmonella typhimurium.[1][2]
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:precorrin-4 C11 methyltransferase. Other names in common use include precorrin-3 methylase, and CobM.[3] It is part of the biosynthetic pathway to vitamin B12 in aerobic bacteria.[4]
See also
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes PDB: 1CBF and PDB: 2CBF.
References
- ↑ Thibaut D, Debussche L (1990). "Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid". J. Bacteriol. 172 (10): 5980–90. doi:10.1128/jb.172.10.5980-5990.1990. PMC 526920. PMID 2211521.
- ↑ Roth JR, Lawrence JG, Rubenfield M, Kieffer-Higgins S, Church GM (1993). "Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium". J. Bacteriol. 175 (11): 3303–16. doi:10.1128/jb.175.11.3303-3316.1993. PMC 204727. PMID 8501034.
- ↑ Enzyme 2.1.1.133 at KEGG Pathway Database.
- ↑ Fang, H; Kang, J; Zhang, D (30 January 2017). "Microbial production of vitamin B12: a review and future perspectives". Microbial Cell Factories. 16 (1): 15. doi:10.1186/s12934-017-0631-y. PMC 5282855. PMID 28137297.
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