Phosphoserine phosphatase
| phosphoserine phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.3.3 | ||||||||
| CAS no. | 9025-73-4 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme phosphoserine phosphatase (EC 3.1.3.3) catalyzes the reversible reaction
- O-phospho-L(or D)-serine + H2O L(or D)-serine + phosphate
For example, with L-phosphoserine as substrate the hydrolysis reaction gives L-serine, with orthophosphate as byproduct:[1]
The enzyme was characterised from chicken and rat liver.[1][2] It is also present in humans, where it regulates glycine and D-serine levels.[3] The reaction is the final, irreversible, step in L-serine biosynthesis.[4][5]
The enzyme is classified as a hydrolase, specifically one acting on phosphoric monoester bonds. The systematic name is O-phosphoserine phosphohydrolase.[6]
Structural studies
As of late 2007, 12 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1F5S, PDB: 1J97, PDB: 1L7M, PDB: 1L7N, PDB: 1L7O, PDB: 1L7P, PDB: 1L8L, PDB: 1L8O, PDB: 1NNL, PDB: 2J6Y, PDB: 2J6Z, and PDB: 2J70.
References
- 1 2 Borkenhagen LF, Kennedy EP (1959). "The enzymatic exchange of L-serine with 'O-phospho-L-serine catalyzed by a specific phosphatase". J. Biol. Chem. 234 (4): 849–53. doi:10.1016/S0021-9258(18)70188-9. PMID 13654276.
- ↑ Neuhaus FC, Byrne WL (1959). "Metabolism of phosphoserine. II. Purification and properties of O-phosphoserine phosphatase". J. Biol. Chem. 234 (1): 113–121. doi:10.1016/S0021-9258(18)70346-3. PMID 13610904.
- ↑ Kim, Hye-Yeon; Heo, Yong-Seok; Kim, Jin Hwan; et al. (2002). "Molecular Basis for the Local Conformational Rearrangement of Human Phosphoserine Phosphatase". Journal of Biological Chemistry. 277 (48): 46651–46658. doi:10.1074/jbc.M204866200. PMID 12213811.
- ↑ Pollegioni, Loredano; Campanini, Barbara; Good, Jean-Marc; et al. (2024). "L-serine deficiency: On the properties of the Asn133Ser variant of human phosphoserine phosphatase". Scientific Reports. 14 (1) 12463. doi:10.1038/s41598-024-63164-y. PMC 11139964. PMID 38816452.
- ↑ Liu, Dafeng; Deng, Huashui; Liu, Xinbao; Yao, Wenshuang; Li, Na (2026). "Molecular insights into the functional mechanism of phosphoserine phosphatase SerB2 in Mycobacterium tuberculosis". Frontiers in Microbiology. 17 1863671. doi:10.3389/fmicb.2026.1863671. PMC 13372908. PMID 42466121.
- ↑ Enzyme 3.1.3.3 at KEGG Pathway Database.