Phosphoethanolamine/phosphocholine phosphatase
| Phosphoethanolamine/phosphocholine phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.3.75 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Phosphoethanolamine/phosphocholine phosphatase (EC 3.1.3.75, PHOSPHO1, 3X11A; systematic name phosphoethanolamine phosphohydrolase) is an enzyme highly expressed in mineralizing cells .[1][2] This enzyme is implicated in bone and cartilage formation and catalyses the following two chemical reactions:
In each case, a phosphate group is removed by hydrolysis, giving orthophosphate (Pi) as a byproduct. The enzyme is specific for phosphorylethanolamine and phosphocholine.[3]
The enzyme is a member of the haloacid dehalogenase superfamily. Like other members of this superfamily it requires a metal ion for catalysis, which is usually Mg2+, it is also active in the presence of Co2+ or Mn2+ but exhibits a lower specific activity with these metal ions.[4]
References
- ↑ Houston B, Seawright E, Jefferies D, Hoogland E, Lester D, Whitehead C, Farquharson C (January 1999). "Identification and cloning of a novel phosphatase expressed at high levels in differentiating growth plate chondrocytes". Biochimica et Biophysica Acta. 1448 (3): 500–6. doi:10.1016/s0167-4889(98)00153-0. PMID 9990301.
- ↑ Stewart AJ, Schmid R, Blindauer CA, Paisey SJ, Farquharson C (December 2003). "Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily". Protein Engineering. 16 (12): 889–95. doi:10.1093/protein/gzg126. PMID 14983068.
- ↑ Roberts SJ, Stewart AJ, Sadler PJ, Farquharson C (August 2004). "Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities". The Biochemical Journal. 382 (Pt 1): 59–65. doi:10.1042/bj20040511. PMC 1133915. PMID 15175005.
- ↑ Enzyme 3.1.3.75 at KEGG Pathway Database.
External links
- Phosphoethanolamine/phosphocholine+phosphatase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)