N-methyl nucleosidase
| N-methyl nucleosidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.2.2.25 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
N-methyl nucleosidase (EC 3.2.2.25) is an enzyme that catalyzes the chemical reaction
This hydrolysis reaction is the second step in the biosynthesis of caffeine from the nucleoside, xanthosine.[1] 7-Methylxanthine is subsequently methylated by the enzyme theobromine synthase.[2]
This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The systematic name of this enzyme class is 7-methylxanthosine ribohydrolase. Other names in common use include 7-methylxanthosine nucleosidase, N-MeNase, N-methyl nucleoside hydrolase, and methylpurine nucleosidase.[3]
References
- ↑ Negishi O, Ozawa T, Imagawa H (1988). "N-Methyl nucleosidase from tea leaves". Agric. Biol. Chem. 52: 169–175. doi:10.1271/bbb1961.52.169.
- ↑ Uefuji H, Ogita S, Yamaguchi Y, Koizumi N, Sano H (2003). "Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants". Plant Physiol. 132 (1): 372–80. doi:10.1104/pp.102.019679. PMC 166982. PMID 12746542.
- ↑ Enzyme 3.2.2.25 at KEGG Pathway Database.
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