m7G(5')pppN diphosphatase
| m7G(5')pppN diphosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.6.1.59 | ||||||||
| CAS no. | 82599-75-5 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a m7G(5')pppN diphosphatase or m7G(5')pppN pyrophosphatase (EC 3.6.1.59) is an enzyme that catalyzes the chemical reaction
- 7-methylguanosine 5'-triphospho-5'-polyribonucleotide + H2O 7-methylguanosine 5'-phosphate + 5'-diphospho-polyribonucleotide
Thus, the two substrates of this enzyme are 7-methylguanosine 5'-triphospho-5'-polynucleotide and H2O, whereas its two products are 7-methylguanosine 5'-phosphate and 5'-diphospho-polyribonucleotide.[1][2][3] It is responsible for messenger RNA decapping.
Nomenclature
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is 7-methylguanosine-5'-triphospho-5'-polynucleotide 7-methylguanosine-5'-phosphohydrolase.
Another name in common use is decapase because this enzyme removes the N7-methylguanosine 5-phosphate cap from an mRNA. The process of mRNA decapping controls eukaryotic mRNA degradation.[4]
See also
References
- ↑ Nuss DL, Furuichi Y (May 1977). "Characterization of the m7G(5')pppN-pyrophosphatase activity from HeLa cells". The Journal of Biological Chemistry. 252 (9): 2815–2821. doi:10.1016/S0021-9258(17)40435-2. PMID 16003.
- ↑ Lavers GC (December 1977). "Cleavage of pm7G from mRNA 5' terminal cap structures by pyrophosphatase activity in embryonic chick lens cells". Molecular Biology Reports. 3 (6): 413–420. doi:10.1007/BF00808382. PMID 593271.
- ↑ Nuss DL, Altschuler RE, Peterson AJ (June 1982). "Purification and characterization of the m7G(5')pppN-pyrophosphatase from human placenta". The Journal of Biological Chemistry. 257 (11): 6224–6230. doi:10.1016/S0021-9258(20)65128-6. PMID 6122684.
- ↑ Furuichi Y (2015). "Discovery of m(7)G-cap in eukaryotic mRNAs". Proceedings of the Japan Academy. Series B, Physical and Biological Sciences. 91 (8): 394–409. doi:10.2183/pjab.91.394. PMC 4729855. PMID 26460318.