Isoleucine–tRNA ligase
| Isoleucine–tRNA ligase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 6.1.1.5 | ||||||||
| CAS no. | 9030-96-0 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, an isoleucine–tRNA ligase (EC 6.1.1.5) is an enzyme that catalyzes a two-step reaction in which isoleucine is attached to its corresponding tRNA molecule:
- isoleucine + ATP → isoleucyl-AMP + PPi
- isoleucyl-AMP + tRNAIle → isoleucyl-tRNAIle + AMP
The 3 substrates of this enzyme are ATP, L-isoleucine, and tRNAIle, whereas its 3 products are AMP, diphosphate, and L-isoleucyl-tRNAIle.
This enzyme belongs to the family of ligases, to be specific those forming carbon–oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-isoleucine:tRNAIle ligase (AMP-forming). Other names in common use include isoleucyl-tRNA synthetase, isoleucyl-transfer ribonucleate synthetase, isoleucyl-transfer RNA synthetase, isoleucine-transfer RNA ligase, isoleucine-tRNA synthetase, and isoleucine translase. This enzyme participates in valine, leucine and isoleucine biosynthesis and aminoacyl-tRNA biosynthesis.
Structural studies
As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes PDB: 1FFY, PDB: 1JZQ, PDB: 1JZS, PDB: 1QU2, PDB: 1QU3, PDB: 1UDZ, PDB: 1UE0, PDB: 1WK8, PDB: 1WNY, and PDB: 1WNZ.
References
- ALLEN EH, GLASSMAN E, SCHWEET RS (1960). "Incorporation of amino acids into ribonucleic acid. I. The role of activating enzymes". J. Biol. Chem. 235 (4): 1061–7. doi:10.1016/S0021-9258(18)69479-7. PMID 13792726.
- Berg P, Bergmann FH, Ofengand EJ, Dieckmann M (1961). "The enzymic synthesis of amino acyl derivatives of ribonucleic acid I. The mechanism of leucyl-, valyl-, isoleucyl- and methionyl ribonucleic acid formation". J. Biol. Chem. 236: 1726–1734. doi:10.1016/S0021-9258(19)63293-X.
- Bergmann FH, Berg P, Dieckmann M (1961). "The enzymic synthesis of amino acyl derivatives of ribonucleic acid II. The preparation of leucyl-, valyl-, isoleucyl- and methionyl ribonucleic acid synthetases from Escherichia coli". J. Biol. Chem. 236: 1735–1740. doi:10.1016/S0021-9258(19)63294-1.