Indolepyruvate C-methyltransferase
| Indolepyruvate C-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.47 | ||||||||
| CAS no. | 54576-88-4 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Indolepyruvate C-methyltransferase (EC 2.1.1.47) is an enzyme that catalyzes the chemical reaction
-3-(indol-3-yl)-2-oxobutyric_acid.svg.png)
This is a methylation reaction in which indole-3-pyruvic acid is converted to (R)-3-(indol-3-yl)-2-oxobutyric acid. The methyl group comes from the cofactor, S-adenosyl methionine (SAM), which becomes S-adenosyl-L-homocysteine (SAH).[1][2]
This enzyme is part of the biosynthetic pathway to the indole alkaloid, indolmycin, in Streptomyces griseus[3]
Nomenclature
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine: (indol-3-yl)pyruvate C-methyltransferase. Other names in common use include indolepyruvate methyltransferase, indolepyruvate 3-methyltransferase, indolepyruvic acid methyltransferase, and S-adenosyl-L-methionine:indolepyruvate C-methyltransferase.[4]
References
- ↑ Hornemann, U.; Speedie, M.K.; Hurley, L.H.; Floss, H.G. (1970). "Demonstration of a C-methylating enzyme in cell free extracts of indolmycin-producing Streptomyces griseus". Biochemical and Biophysical Research Communications. 39 (4): 594–599. doi:10.1016/0006-291X(70)90245-7. PMID 5490210.
- ↑ Du, Yi-Ling; Alkhalaf, Lona M.; Ryan, Katherine S. (2015). "In vitro reconstitution of indolmycin biosynthesis reveals the molecular basis of oxazolinone assembly". Proceedings of the National Academy of Sciences. 112 (9): 2717–2722. doi:10.1073/pnas.1419964112. PMC 4352800. PMID 25730866.
- ↑ Hornemann, U.; Hurley, L. H.; Speedie, M. K.; Floss, H. G. (1971). "Biosynthesis of indolmycin". Journal of the American Chemical Society. 93 (12): 3028–3035. doi:10.1021/ja00741a035. PMID 5095271.
- ↑ Enzyme 2.1.1.47 at KEGG Pathway Database.
Categories: