HROB (gene)
| HROB | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Identifiers | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Aliases | HROB, chromosome 17 open reading frame 53, C17orf53, MCM8IP, homologous recombincation factor with OB-fold, homologous recombination factor with OB-fold | |||||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | MGI: 2387601; GeneCards: HROB | |||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Wikidata | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Homologous recombination OB-fold protein is a protein which in humans is encoded by the gene HROB (previously C17orf53).[5] It is involved in the repair of DNA, and is most highly expressed in the digestive system and female reproductive system.[5] Pathogenic variants have been associated with female reproductive system dysfunction, including premature menopause and ovarian dysgenesis.[5]
Gene
Gene neighborhood
Neighboring genes of HROB are that of RNU6-131, RNA U6 Small Nuclear 131 Pseudogene, and ASB16, Ankyrin Repeat And SOCS Box Containing 16.[6][7][8]
Expression
HROB has been observed to be expressed ubiquitously across almost all tissue types of the body, but with highest expression found in the duodenum and fallopian tubes, while in the brain there was little region specificity. However, inconsistencies between antibody staining and RNA expression make interpretation of these results difficult.[9]
Transcript variants
The coding region of HROB consists of 2699 base pairs and encodes for a protein that is 647 amino acids long.[10] Per NCBI AceView, the transcription of HROB produces nine alternatively spliced mRNAs and 17 distinct gt-ag introns[11] Of these nine alternatively spliced variants four distinct protein products are formed.[12]
| Name | Reference Sequence Number | Length (aa) | Mass (Da) |
|---|---|---|---|
| Q8N3J3-1 | NM_024032.4 | 647 | 69,771 |
| Q8N3J3-2 | NM_001171251.2 | 137 | 14,269 |
| Q8N3J3-3 | NM_001321310.1 | 521 | 56,181 |
| Q8N3J3-4 | NM_001321311.1 | 646 | 69,643 |
Protein
General properties
The molecular weight of HROB is 69 kilodaltons.[12] The isoelectric point is 5.85.[13] The protein sequence of HROB is both Proline and Glutamine rich, while low in Tyrosine.[13] Aside from Proline, Glutamine, and Tyrosine, there exists a relatively even distribution of amino acids in the protein product of HROB.
Subcellular localization
The protein product of HROB has been shown to target the nucleus, with minor localization in the cytoplasm.[5]
Interacting proteins
- MCM8, MCM9 — HROB interacts with the MCM8-MCM9 helicase complex, which is involved in DNA repair. In particular, it helps recruit the complex to areas of damaged DNA.[14]
- RPA1 — HROB interacts with the heterotrimeric RPA complex via its interaction with RPA1.[14] This complex helps stabilize DNA while it’s being repaired.[15]
References
- 1 2 3 GRCh38: Ensembl release 89: ENSG00000125319 – Ensembl, May 2017
- 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000034773 – Ensembl, May 2017
- ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- 1 2 3 4 "HROB gene Homologous Recombination Factor With OB-Fold". genecards.org. LifeMap Sciences, Inc. Retrieved 2026-07-26.
- 1 2 "C17orf53 chromosome 17 open reading frame 53 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2018-04-27.
- ↑ "RNU6-131P Gene". www.genecards.org. Retrieved 2018-05-06.
- ↑ "ASB16 Gene". www.genecards.org. Retrieved 2018-05-06.
- ↑ "HROB". www.proteinatlas.org. Retrieved 2026-07-26.
- ↑ "Homo sapiens chromosome 17 open reading frame 53 (C17orf53), transcrip - Nucleotide - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2018-05-06.
- ↑ Thierry-Mieg D, Thierry-Mieg J. "AceView: Gene:C17orf53, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView". www.ncbi.nlm.nih.gov. Retrieved 2018-02-20.
- 1 2 3 "C17orf53 - Uncharacterized protein C17orf53 - Homo sapiens (Human) - C17orf53 gene & protein". UniProt. Retrieved 2018-05-06.
- 1 2 EMBL-EBI. "SAPS < Sequence Statistics < EMBL-EBI". www.ebi.ac.uk. Retrieved 2018-05-06.
- 1 2 "Q8N3J3: Hrob_human". UniProt. Retrieved 2026-07-26.
- ↑ Sciences L, Team TG. "RPA1 gene Replication Protein A1". www.genecards.org. Retrieved 2026-07-26.