GRP RNA motif
The GRP RNA motif is a conserved RNA structural element identified in introns of glycine-rich RNA-binding protein genes, including GRP7 and GRP8. It is associated with alternative splicing and nonsense-mediated decay (NMD). GRP RNA motif has been reported as a cis-regulatory element, that modulates binding of GRP7 and GRP8 proteins to their RNA targets.[1]
Structure
The motif is predicted to form a stem-loop structure of approximately 20 base pairs. An alternative 5′ splice site is located near the base of the stem, suggesting that RNA structure may influence splice-site accessibility.[1]
GRP proteins function
In Arabidopsis thaliana (At), AtGRP7 and AtGRP8 are circadian clock-regulated RNA-binding proteins that form an interlocked auto-regulatory and cross-regulatory feedback network based on alternative splicing and NMD of their own pre-mRNAs, contributing to circadian output regulation, were transcript levels oscillate with a daily rhythm.[2] GRP7 and GRP8 are also involved in regulating flowering time.[3][4][5]
Distribution
Homologous motifs of GRP RNA motif have been identified in multiple plant species within the Pentapetalae clade.[1]
References
- 1 2 3 Sack, Maximilian; Reinhardt, Maren; Burgardt, Rica; Berg, Philipp; Bauer, Julia; Wachter, Andreas; Weinberg, Zasha (2025). "Identification and characterization of new structured RNA classes in plants". RNA biology. 22 (1): 1–16. doi:10.1080/15476286.2025.2523696. ISSN 1555-8584. PMC 12218528. PMID 40587820.
- ↑ Schöning, Jan C.; Streitner, Corinna; Meyer, Irmtraud M.; Gao, Yahong; Staiger, Dorothee (2008). "Reciprocal regulation of glycine-rich RNA-binding proteins via an interlocked feedback loop coupling alternative splicing to nonsense-mediated decay in Arabidopsis". Nucleic Acids Research. 36 (22): 6977–6987. doi:10.1093/nar/gkn847. ISSN 1362-4962. PMC 2602770. PMID 18987006.
- ↑ Staiger, Dorothee; Zecca, Laura; Wieczorek Kirk, Dominika A.; Apel, Klaus; Eckstein, Luca (2003). "The circadian clock regulated RNA-binding protein AtGRP7 autoregulates its expression by influencing alternative splicing of its own pre-mRNA". The Plant Journal: For Cell and Molecular Biology. 33 (2): 361–371. doi:10.1046/j.1365-313x.2003.01629.x. ISSN 0960-7412. PMID 12535349.
- ↑ Heintzen, Christian; Nater, Mena; Apel, Klaus; Staiger, Dorothee (1997-08-05). "AtGRP7, a nuclear RNA-binding protein as a component of a circadian-regulated negative feedback loop in Arabidopsis thaliana". Proceedings of the National Academy of Sciences. 94 (16): 8515–8520. doi:10.1073/pnas.94.16.8515. PMC 22981. PMID 9238008.
- ↑ Steffen, Alexander; Elgner, Mareike; Staiger, Dorothee (2019-09-01). "Regulation of Flowering Time by the RNA-Binding Proteins AtGRP7 and AtGRP8". Plant and Cell Physiology. 60 (9): 2040–2050. doi:10.1093/pcp/pcz124. ISSN 0032-0781.