Fluoroacetyl-CoA thioesterase
| Fluoroacetyl-CoA thioesterase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.2.29 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
The enzyme fluoroacetyl-CoA thioesterase (EC 3.1.2.29; systematic name fluoroacetyl-CoA hydrolase) catalyses the following reaction:
The enzyme characterised from the plant Dichapetalum cymosum and the bacterium Streptomyces cattleya hydrolyses fluoroacetyl-CoA to coenzyme A and fluoroacetic acid, which form the thioester in the starting material.[1][2][3]
Dichapetalum cymosum is native to northern parts of Southern Africa and is a common cause of lethal cattle poisoning owing to the content of fluoroacetic acid in its leaves.[4] Fluoroacetate is extremely toxic to most organisms. The enzyme ensures that any fluoroacetyl-CoA produced by normal metabolic processes that use acetyl-CoA cannot go on to be incorporated into fluorocitric acid by citrate synthase.[5]
References
- ↑ Meyer JJ, Grobbelaar N, Vleggaar R, Louw AI (1992). "Fluoroacetyl-coenzyme-A hydrolase-like activity in Dichapetalum cymosum". J. Plant Physiol. 139 (3): 369–372. Bibcode:1992JPPhy.139..369M. doi:10.1016/s0176-1617(11)80352-4.
- ↑ Huang F, Haydock SF, Spiteller D, Mironenko T, Li TL, O'Hagan D, Leadlay PF, Spencer JB (May 2006). "The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A". Chemistry & Biology. 13 (5): 475–84. doi:10.1016/j.chembiol.2006.02.014. PMID 16720268.
- ↑ Dias MV, Huang F, Chirgadze DY, Tosin M, Spiteller D, Dry EF, Leadlay PF, Spencer JB, Blundell TL (July 2010). "Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK". The Journal of Biological Chemistry. 285 (29): 22495–504. doi:10.1074/jbc.m110.107177. PMC 2903362. PMID 20430898.
- ↑ Kellerman, T.S.; Naude, T.W.; Fourie, N.; Verwoerd, D.W. (1996). "The distribution, diagnoses and estimated economic impact of plant poisonings and mycotoxicosis in South Africa". Onderstepoort Journal of Veterinary Research. 63 (2): 65–90. hdl:2263/42595. PMID 8856758.
- ↑ Chandra, Girish; Mahato, Gopal Kumar; Mahto, Birkishore (2024). "Biosynthesis and Synthetic Biology of Fluorinated Products in Plants". Biosynthesis of Natural Products in Plants. pp. 381–403. doi:10.1007/978-981-97-2166-5_16. ISBN 978-981-97-2165-8.
External links
- Fluoroacetyl-CoA+thioesterase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)