Erythromycin 3''-O-methyltransferase
| Erythromycin 3''-O-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.254 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Erythromycin 3''-O-methyltransferase (EC 2.1.1.254, EryG) is an enzyme with systematic name S-adenosyl-L-methionine:erythromycin C 3''-O-methyltransferase.[1] This enzyme catalyses two related chemical reactions. The first is the final step in the biosynthesis of the antibiotic, erythromycin A:
This is a methylation reaction at the 3-position of the mycarose group; the methyl group comes from the cofactor, S-adenosyl methionine (SAM), which becomes S-adenosyl-L-homocysteine (SAH). The enzyme was characterised from Saccharopolyspora erythraea.[2]
The enzyme can also convert erythromycin D to erythromycin B.[3]
erythromycin D
erythromycin B
References
- ↑ Paulus TJ, Tuan JS, Luebke VE, Maine GT, DeWitt JP, Katz L (May 1990). "Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli". Journal of Bacteriology. 172 (5): 2541–6. doi:10.1128/jb.172.5.2541-2546.1990. PMC 208895. PMID 2185226.
- ↑ Summers RG, Donadio S, Staver MJ, Wendt-Pienkowski E, Hutchinson CR, Katz L (October 1997). "Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production". Microbiology. 143 (10): 3251–62. doi:10.1099/00221287-143-10-3251. PMID 9353926.
- ↑ Enzyme 2.1.1.254 at KEGG Pathway Database.
External links
- Erythromycin+3''-O-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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