DPPH radical scavenging assay

DPPH radical scavenging assay is a method for determining antioxidant^[1] radical scavenging^[2] on 2,2-diphenyl-1-picrylhydrazyl (DPPH) by spectrophotometric measurement.^[3] The method was developed by Marsden Blois and reported in April 1958.^[3]^[4]

DPPH radicals were discovered during 1922 by Goldschmidt and Renn^[3] which they described as α,α-diphenyl-β-trinitrophenyl-hydrazyl.^[5] DPPH absorbs at 515^[1] 517 nanometers.^[2]

References

1 2 W. Brand-Williams; M.E. Cuvelier; C. Berset (1995). "Use of a free radical method to evaluate antioxidant activity". LWT - Food Science and Technology. 28 (1): 25–30. Bibcode:1995LWTFS..28...25B. doi:10.1016/S0023-6438(95)80008-5 – via Daniel Załuski et al. (July 2017) LC-ESI-MS/MS profiling of phenolics from Eleutherococcus spp. inflorescences, structure-activity relationship as antioxidants, inhibitors of hyaluronidase and acetylcholinesterase Saudi Pharmaceutical Journal Volume 25, Issue 5.
1 2 Siddartha Baliyan; et al. (16 February 2022). Fawzy A Elbarbry; Deepa A Rao (eds.). "Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa". Molecules. 27 (4). MDPI: 1326. doi:10.3390/molecules27041326. PMC 8878429. PMID 35209118.
1 2 3 İlhami Gulcin; Saleh H. Alwasel (2023). "DPPH Radical Scavenging Assay - 4. What Are DPPH Radicals?". Processes. 11 (8). King Saud University: MDPI. doi:10.3390/pr11082248.
↑ MARSDEN S. BLOIS (26 April 1958). "Antioxidant Determinations by the Use of a Stable Free Radical". Nature. 181 (4617). W. W. Hansen Laboratories of Physics, Stanford University, California: SPRINGER NATURE: 1199–1200. Bibcode:1958Natur.181.1199B. doi:10.1038/1811199a0. ISSN 1476-4687 – via Gulcin & Alwasel (2023).
↑ Stefan Goldschmidt; Konard Renn (11 March 1922). "Zweiwertiger Stickstoff: Über das α, α-Diphenyl-β-trinitrophenyl-hydrazyl. (IV. Mitteilung über Amin-Oxydation)". Berichte der deutschen chemischen Gesellschaft (A and B Series). 55 (3). European Chemical Societies Publishing: Wiley: 628–643. doi:10.1002/cber.19220550308.

[WBW80008-1] 1 2 W. Brand-Williams; M.E. Cuvelier; C. Berset (1995). "Use of a free radical method to evaluate antioxidant activity". LWT - Food Science and Technology. 28 (1): 25–30. Bibcode:1995LWTFS..28...25B. doi:10.1016/S0023-6438(95)80008-5 – via Daniel Załuski et al. (July 2017) LC-ESI-MS/MS profiling of phenolics from Eleutherococcus spp. inflorescences, structure-activity relationship as antioxidants, inhibitors of hyaluronidase and acetylcholinesterase Saudi Pharmaceutical Journal Volume 25, Issue 5.

[SB27041326-2] 1 2 Siddartha Baliyan; et al. (16 February 2022). Fawzy A Elbarbry; Deepa A Rao (eds.). "Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa". Molecules. 27 (4). MDPI: 1326. doi:10.3390/molecules27041326. PMC 8878429. PMID 35209118.

[İG11082248-3] 1 2 3 İlhami Gulcin; Saleh H. Alwasel (2023). "DPPH Radical Scavenging Assay - 4. What Are DPPH Radicals?". Processes. 11 (8). King Saud University: MDPI. doi:10.3390/pr11082248.

[4] MARSDEN S. BLOIS (26 April 1958). "Antioxidant Determinations by the Use of a Stable Free Radical". Nature. 181 (4617). W. W. Hansen Laboratories of Physics, Stanford University, California: SPRINGER NATURE: 1199–1200. Bibcode:1958Natur.181.1199B. doi:10.1038/1811199a0. ISSN 1476-4687 – via Gulcin & Alwasel (2023).

[5] Stefan Goldschmidt; Konard Renn (11 March 1922). "Zweiwertiger Stickstoff: Über das α, α-Diphenyl-β-trinitrophenyl-hydrazyl. (IV. Mitteilung über Amin-Oxydation)". Berichte der deutschen chemischen Gesellschaft (A and B Series). 55 (3). European Chemical Societies Publishing: Wiley: 628–643. doi:10.1002/cber.19220550308.

[1]

[2]

[3]

[4]

[5]