Cytosol nonspecific dipeptidase

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Cytosol nonspecific dipeptidase
Cytosol nonspecific dipeptidase
Identifiers
EC no.	3.4.13.18
CAS no.	9025-31-4
Databases
BRENDA	enzyme data
ExPASy	NiceZyme view
KEGG	enzyme entry
MetaCyc	metabolic pathway
Rhea	reactions
PDB structures	RCSB PDB PDBe PDBsum
PMC
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PMC	articles
PubMed	articles
NCBI	proteins

CNDP2
Identifiers
Aliases	CNDP2, CN2, CPGL, HEL-S-13, HsT2298, PEPA, CNDP dipeptidase 2 (metallopeptidase M20 family), carnosine dipeptidase 2
External IDs	OMIM: 169800; MGI: 1913304; GeneCards: CNDP2
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	4RUH
Enzyme activity
Gene location (Human)
Chr.	Chromosome 18 (human)
End	74,523,454 bp
Gene location (Mouse)
Chr.	Chromosome 18 (mouse)
End	84,703,827 bp
RNA expression pattern
	Top expressed in
	kidney tubule; ; renal medulla; ; endothelial cell; ; human kidney; ; mucosa of ileum; ; glomerulus; ; metanephric glomerulus; ; amniotic fluid; ; jejunal mucosa; ; corpus callosum;
	Top expressed in
	vestibular membrane of cochlear duct; ; right kidney; ; proximal tubule; ; Paneth cell; ; vestibular sensory epithelium; ; stroma of bone marrow; ; jejunum; ; human kidney; ; internal carotid artery; ; ileum;
	More reference expression data
	n/a
Gene ontology
Molecular function	carboxypeptidase activity; peptidase activity; hydrolase activity; metallopeptidase activity; dipeptidase activity; metal ion binding; alanylglutamate dipeptidase activity;
Cellular component	extracellular exosome; nucleoplasm; cytoplasm; cytosol;
Biological process	metabolism; glutathione biosynthetic process; proteolysis;
	Sources:Amigo / QuickGO
Orthologs
Databases	NCBI: entry; OMA: entry
	55748
	66054
	ENSG00000133313
	ENSMUSG00000024644
	Q96KP4
	Q9D1A2
NM_001168499; NM_018235; NM_001370248; NM_001370249; NM_001370250;
	NM_001370254
	NM_001289531; NM_023149
NP_001161971; NP_060705; NP_001357177; NP_001357178; NP_001357179;
	NP_001357183
	NP_001276460; NP_075638
	Wikidata
View/Edit Human	View/Edit Mouse

Cytosolic non-specific dipeptidase (EC 3.4.13.18) also known as carnosine dipeptidase 2 is an enzyme that in humans is encoded by the CNDP2 gene.^[5]^[6]^[7] This enzyme catalyses the hydrolysis of dipeptides, preferentially those containing hydrophobic amino acids. The human enzyme uses manganese ions as a cofactor.^[8] In addition to its function as a peptidase, the enzyme also functions to generate N-lactoyl amino acids, such as N-lactoyl-phenylalanine, via the process of "reverse proteolysis".^[9] A similar pathway conjugates amino acids to Beta-hydroxybutyric acid.^[10]

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000133313 – Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000024644 – Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Teufel M, Saudek V, Ledig JP, Bernhardt A, Boularand S, Carreau A, et al. (February 2003). "Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase". The Journal of Biological Chemistry. 278 (8): 6521–6531. doi:10.1074/jbc.M209764200. PMID 12473676.
↑ "Entrez Gene: CNDP2 carnosine dipeptidase 2".
↑ Bauer K (1998). "Cytosol non-specific dipeptidase". In Barrett AJ, Rawlings ND, Woessner JF (eds.). Handbook of Proteolytic Enzymes. London: Academic Press. pp. 1520–1522.
↑ "UniProt: CDNP2 - Cytosolic non-specific dipeptidase: Homo sapiens".
↑ Jansen RS, Addie R, Merkx R, Fish A, Mahakena S, Bleijerveld OB, et al. (May 11, 2015). "N-lactoyl-amino acids are ubiquitous metabolites that originate from CDNP2-mediated reverse proteolysis of lactate and amino acids". Proceedings of the National Academy of Sciences of the USA. 112 (21): 6601–6606. Bibcode:2015PNAS..112.6601J. doi:10.1073/pnas.1424638112. PMC 4450436. PMID 25964343.
↑ Moya-Garzon MD, Wang M, Li VL, Lyu X, Wei W, Tung AS, et al. (2024). "A β-hydroxybutyrate shunt pathway generates anti-obesity ketone metabolites". Cell. 188 (1): 175–186.e20. doi:10.1016/j.cell.2024.10.032. PMC 11724754. PMID 39536746.{{cite journal}}: CS1 maint: multiple names: authors list (link)

External links

Cytosol+non-specific+dipeptidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000133313 – Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000024644 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid12473676-5] Teufel M, Saudek V, Ledig JP, Bernhardt A, Boularand S, Carreau A, et al. (February 2003). "Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase". The Journal of Biological Chemistry. 278 (8): 6521–6531. doi:10.1074/jbc.M209764200. PMID 12473676.

[entrez-6] "Entrez Gene: CNDP2 carnosine dipeptidase 2".

[7] Bauer K (1998). "Cytosol non-specific dipeptidase". In Barrett AJ, Rawlings ND, Woessner JF (eds.). Handbook of Proteolytic Enzymes. London: Academic Press. pp. 1520–1522.

[8] "UniProt: CDNP2 - Cytosolic non-specific dipeptidase: Homo sapiens".

[9] Jansen RS, Addie R, Merkx R, Fish A, Mahakena S, Bleijerveld OB, et al. (May 11, 2015). "N-lactoyl-amino acids are ubiquitous metabolites that originate from CDNP2-mediated reverse proteolysis of lactate and amino acids". Proceedings of the National Academy of Sciences of the USA. 112 (21): 6601–6606. Bibcode:2015PNAS..112.6601J. doi:10.1073/pnas.1424638112. PMC 4450436. PMID 25964343.

[pmid39536746-10] Moya-Garzon MD, Wang M, Li VL, Lyu X, Wei W, Tung AS, et al. (2024). "A β-hydroxybutyrate shunt pathway generates anti-obesity ketone metabolites". Cell. 188 (1): 175–186.e20. doi:10.1016/j.cell.2024.10.032. PMC 11724754. PMID 39536746.{{cite journal}}: CS1 maint: multiple names: authors list (link)

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Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

See also

References

External links