Alpha-ribazole phosphatase
| adenosylcobalamin/α-ribazole phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.3.73 | ||||||||
| CAS no. | 251991-06-7 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The primary biochemical reaction catalyzed by the enzyme adenosylcobalamin/α-ribazole phosphatase (formerly α-ribazole phosphatase)[1] (EC 3.1.3.73) is
- adenosylcobalamin 5′-phosphate + H2O = coenzyme B12 + phosphate
This enzyme can also catalyze the following reaction in vitro, however it is not the biologically relevant reaction
- α-ribazole 5′-phosphate + H2O α-ribazole + phosphate
This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name is adenosylcobalamin/α-ribazole-5′-phosphate phosphohydrolase. This enzyme is also called CobC. The reaction is the final step in the biosynthetic pathway to cobalamin (vitamin B12) in bacteria.[2][3]
Structural studies
As of late 2007, 16 structures have been solved for this class of enzymes, with PDB accession codes PDB: 2ENU, PDB: 2ENW, PDB: 2EOA, PDB: 2OWE, PDB: 2P2Y, PDB: 2P2Z, PDB: 2P30, PDB: 2P6M, PDB: 2P6O, PDB: 2P75, PDB: 2P77, PDB: 2P78, PDB: 2P79, PDB: 2P9Y, PDB: 2P9Z, and PDB: 2PA0.
See also
References
- ↑ O'Toole GA, Trzebiatowski JR, Escalante-Semerena JC (1994). "The cobC gene of Salmonella typhimurium codes for a novel phosphatase involved in the assembly of the nucleotide loop of cobalamin". J. Biol. Chem. 269 (42): 26503–11. doi:10.1016/S0021-9258(18)47223-7. PMID 7929373.
- ↑ Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC (2002). "The biosynthesis of adenosylcobalamin (vitamin B12)". Nat. Prod. Rep. 19 (4): 390–412. doi:10.1039/b108967f. PMID 12195810.
- ↑ Zayas CL, Escalante-Semerena JC (2007). "Reassessment of the late steps of coenzyme B12 synthesis in Salmonella enterica: evidence that dephosphorylation of adenosylcobalamin-5′-phosphate by the CobC phosphatase is the last step of the pathway". J. Bacteriol. 189 (6): 2210–8. doi:10.1128/jb.01665-06. PMC 1899380. PMID 17209023.