Aerobactin synthase
| Aerobactin synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 6.3.2.39 | ||||||||
| CAS no. | 94047-30-0 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Aerobactin synthase (EC 6.3.2.39) is an enzyme that catalyzes the chemical reaction
- 4 ATP + citrate + 2 N6-acetyl-N6-hydroxy-L-lysine + 2 H2O 4 ADP + 4 phosphate + aerobactin
The enzyme from Aerobacter aerogenes combines two units of a lysine-derived compound with citric acid, driven by the energy produced by the hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP):[1][2]
-acetyl-N(6)-hydroxy-L-lysine.svg.png)
The product, aerobactin, is a siderophore that chelates iron, allowing uptake of that element into bacteria.[3][4]
The enzyme has also been characterised from Escherichia coli[5] and belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (ADP-forming). It is also called citrate:6-N-acetyl-6-N-hydroxy-L-lysine ligase (ADP-forming).[6]
References
- ↑ Gibson F, Magrath DI (1969). "The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I". Biochim. Biophys. Acta. 192 (2): 175–84. doi:10.1016/0304-4165(69)90353-5. PMID 4313071.
- ↑ Appanna DL, Grundy BJ, Szczepan EW, Viswanatha T (1984). "Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1". Biochim. Biophys. Acta. 801 (3): 437–443. doi:10.1016/0304-4165(84)90150-8.
- ↑ Maurer PJ, Miller M (1982). "Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine". J. Am. Chem. Soc. 104 (11): 3096–3101. Bibcode:1982JAChS.104.3096M. doi:10.1021/ja00375a025.
- ↑ Challis GL (2005). "A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases". ChemBioChem. 6 (4): 601–11. doi:10.1002/cbic.200400283. PMID 15719346.
- ↑ de Lorenzo V, Bindereif A, Paw BH, Neilands JB (1986). "Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12". J. Bacteriol. 165 (2): 570–8. doi:10.1128/jb.165.2.570-578.1986. PMC 214457. PMID 2935523.
- ↑ Enzyme 6.3.2.39 at KEGG Pathway Database.