Acetoacetyl-CoA hydrolase
| Acetoacetyl-CoA hydrolase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.2.11 | ||||||||
| CAS no. | 37288-10-1 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme acetoacetyl-CoA hydrolase (EC 3.1.2.11) catalyzes the reaction
The enzyme characterised from liver hydrolyses acetoacetyl-CoA to coenzyme A and acetoacetic acid, which forms the thioester in the starting material.[1][2][3] The enzyme from Thermosipho melanesiensis has been studied as a means of producing acetone by metabolic engineering.[4]
This is a hydrolase, specifically one acting on thioester bonds. The systematic name is acetoacetyl-CoA hydrolase. Other names in common use include acetoacetyl coenzyme A hydrolase, acetoacetyl CoA deacylase, and acetoacetyl coenzyme A deacylase.[5]
References
- ↑ Drummond GI, Stern JR (1960). "Enzymes of ketone body metabolism. II. Properties of an acetoacetate-synthesizing enzyme prepared from ox liver". J. Biol. Chem. 235 (2): 318–25. doi:10.1016/S0021-9258(18)69522-5. PMID 13818236.
- ↑ Aragon JJ, Lowenstein JM (1983). "A survey of enzymes which generate or use acetoacetyl thioesters in rat liver". J. Biol. Chem. 258 (8): 4725–33. doi:10.1016/S0021-9258(18)32482-7. PMID 6131897.
- ↑ Schomburg, Dietmar; Salzmann, Margit (1991). "Acetoacetyl-CoA hydrolase". Enzyme Handbook 3. pp. 261–263. doi:10.1007/978-3-642-76463-9_58. ISBN 978-3-642-48962-4.
- ↑ Bing, Ryan G.; Buhrman, Greg K.; Ford, Kathryne C.; et al. (2025). "Structural and kinetic characterization of an acetoacetyl-Coenzyme A: Acetate Coenzyme a transferase from the extreme thermophile Thermosipho melanesiensis". Biochemical Journal. 482 (4): 225–240. doi:10.1042/BCJ20240747. PMC 12400318. PMID 39869497.
- ↑ Enzyme 3.1.2.11 at KEGG Pathway Database.
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