5-phytase
| 5-phytase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.3.72 | ||||||||
| CAS no. | 357208-41-4 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme 5-phytase (EC 3.1.3.72) catalyzes the reaction
The enzyme characterised from Lilium longiflorum pollen hydrolyses phytic acid (myo-inositol hexakisphosphate) to 1L-myo-inositol 1,2,3,4,6-pentakisphosphate and orthophosphate (Pi). Mechanistically, this is a histidine acid phosphatase.[1][2] A protein tyrosine phosphatase-like phytase from Selenomonas ruminantium subsp. lactilytica has also been described. Both are specific for the 5-phosphate position of myo-inositol hexakisphosphate.[3][4]
This enzyme is a hydrolase, specifically one acting on phosphoric monoester bonds. The systematic name of this enzyme class is myo-inositol-hexakisphosphate 5-phosphohydrolase.[5]
Structural studies
As of late 2007, only the phytase purified from lily pollen had its structure solved, with PDB accession codes PDB: 1U24, PDB: 1U25, PDB: 1U26, PDB: 2B4O, PDB: 2B4P, and PDB: 2B4U.
See also
- 3-phytase (1-phytase)
- 4-phytase (6-phytase)
- Protein tyrosine phosphatase
References
- ↑ Barrientos L, Scott JJ, Murthy PP (1994). "Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen". Plant Physiology. 106 (4): 1489–1495. doi:10.1104/pp.106.4.1489. PMC 159689. PMID 7846160.
- ↑ Gocheva, Yana; Engibarov, Stephan; Lazarkevich, Irina; Eneva, Rumyana (2023). "Phytases - Types, Sources, and Factors Affecting Their Activity" (PDF). Acta Microbiologica Bulgaria. 39 (3).
- ↑ Puhl A, Greiner R, Selinger LB (2008). "A protein tyrosine phosphatase-like inositol polyphosphatase from Selenomonas ruminantium subsp. lactilytica has specificity for the 5-phosphate of myo-inositol hexakisphosphate". The International Journal of Biochemistry & Cell Biology. 40 (10): 2053–2064. doi:10.1016/j.biocel.2008.02.003. PMID 18358762.
- ↑ Nezhad, Nima Ghahremani; Rahman, Raja Noor Zaliha Raja Abd; Normi, Yahaya M.; Oslan, Siti Nurbaya; Shariff, Fairolniza Mohd; Leow, Thean Chor (2023). "Isolation, screening and molecular characterization of phytase-producing microorganisms to discover the novel phytase". Biologia. 78 (9): 2527–2537. doi:10.1007/s11756-023-01391-w.
- ↑ Enzyme 3.1.3.72 at KEGG Pathway Database.