| Tie |
Home team (tier) |
Score |
Away team (tier) |
Att. |
| Friday 31 August 2018 |
| 5 | Whickham (9) | 1–0 | Jarrow (10) | 230 |
| 35 | AFC Emley (10) | 3–4 | Litherland REMYCA (9) | 199 |
| 95 | Walsham-le-Willows (9) | 3–0 | Diss Town (10) | 179 |
| 103 | Wormley Rovers (10) | P–P | Biggleswade United (9) | - |
| Match abandoned after injury to Wormley Rovers player before kick-off. Match played at Biggleswade United. |
| 150 | Bagshot (10) | 0–5 | Sidlesham (10) | 70 |
| 152 | Lingfield (9) | 6–1 | Epsom & Ewell (10) | 91 |
| 188 | Christchurch (9) | 3–0 | Folland Sports (10) | 81 |
| Saturday 1 September 2018 |
| 1 | Alnwick Town (11) | 1–2 | Charnock Richard (9) | 113 |
| 2 | Stokesley Sports Club (11) | 0–9 | Guisborough Town (9) | 150 |
| 3 | Holker Old Boys (10) | 0–2 | Prestwich Heys (10) | 26 |
| 4 | Goole (9) | 1–2 | Barnoldswick Town (9) | 100 |
| 6 | West Allotment Celtic (10) | 3–0 | Yorkshire Amateur (9) | 44 |
| 7 | Darlington Railway Athletic (11) | 0–5 | Bedlington Terriers (10) | 35 |
| 8 | Chester-le-Street Town (10) | 1–5 | Ryhope Colliery Welfare (9) | 116 |
| 9 | Esh Winning (10) | 0–6 | Whitley Bay (9) | 130 |
| 10 | Bishop Auckland (9) | 2–2 | Newton Aycliffe (9) | 394 |
| 11 | North Shields (9) | 1–2 | Consett (9) | 279 |
| 12 | Daisy Hill (10) | 5–2 | Whitehaven (11) | 36 |
| 13 | Harrogate Railway Athletic (9) | 3–4 | Silsden (9) | 100 |
| 14 | Thackley (9) | 3–2 | Thornaby (10) | 110 |
| 15 | Dunston UTS (9) | 2–0 | Heaton Stannington (10) | 220 |
| 16 | Team Northumbria (9) | W.O. | Garforth Town (9) | - |
| Walkover for Garforth Town as Team Northumbria were removed from the competition. |
| 17 | Billingham Town (10) | 2–0 | Campion (10) | 188 |
| 18 | Blyth (9) | W.O. | Crook Town (10) | - |
| Walkover for Crook Town as Blyth were removed from the tournament as the club folded. |
| 19 | Billingham Synthonia (10) | 3–1 | Knaresborough Town (9) | 85 |
| 20 | Durham City (10) | 0–5 | Seaham Red Star (9) | 78 |
| 21 | Eccleshill United (9) | 0–1 | Hebburn Town (9) | 111 |
| 22 | Northallerton Town (10) | 2–2 | Willington (10) | 121 |
| 23 | Lower Breck (10) | 3–1 | West Didsbury & Chorlton (9) | 101 |
| 24 | Longridge Town (10) | 1–3 | Vauxhall Motors (10) | 116 |
| 25 | Worksop Town (9) | 2–1 | Grimsby Borough (10) | 374 |
| 26 | Burscough (9) | 5–1 | Alsager Town (10) | 68 |
| 27 | Wythenshawe Town (10) | – | Rylands (10) | - |
| Both Wythenshawe Town and Rylands were not accepted into the competition. |
| 28 | Winsford United (9) | 2–1 (a.e.t.) | Liversedge (9) | 92 |
| 29 | Maltby Main (9) | 5–1 | Ashton Athletic (9) | 80 |
| 30 | Ashton Town (10) | 3–1 | St. Helens Town (10) | 57 |
| 31 | Barton Town (9) | 3–1 | AFC Liverpool (10) | 139 |
| 32 | AFC Blackpool (10) | 3–1 | Nostell Miners Welfare (10) | 62 |
| 33 | Sandbach United (10) | 5–1 | Athersley Recreation (9) | 104 |
| 34 | Shelley (10) | 2–1 | Penistone Church (9) | 225 |
| 36 | Swallownest (10) | 1–0 | Abbey Hey (9) | 128 |
| 37 | Cammell Laird 1907 (10) | 2–1 | Harworth Colliery (10) | 58 |
| 38 | Armthorpe Welfare (10) | 1–2 | Worsbrough Bridge Athletic (10) | 78 |
| 39 | Parkgate (10) | 5–2 (a.e.t.) | Chadderton (10) | 35 |
| 40 | Congleton Town (9) | 1–2 | Bootle (9) | 150 |
| 41 | Selby Town (10) | 0–5 | Staveley Miners Welfare (9) | 135 |
| 42 | Kirby Muxloe (9) | 1–2 | Brocton (10) | 42 |
| 43 | Nuneaton Griff (10) | 3–1 | Coton Green (11) | 45 |
| 44 | Littleton (10) | 1–0 | Lichfield City (10) | 47 |
| 45 | Coventry Sphinx (9) | 2–2 | Sporting Khalsa (9) | 92 |
| 47 | Abbey Hulton United (10) | 2–3 | Whitchurch Alport (9) | 159 |
| 48 | AFC Bridgnorth (10) | 1–3 | Wolverhampton Casuals (10) | 36 |
| 49 | Ellesmere Rangers (10) | 0–4 | Birstall United (10) | 63 |
| 50 | Rugby Borough (11) | 3–2 | Malvern Town (10) | 106 |
| 51 | Stafford Town (11) | 2–3 | Wednesfield (10) | 40 |
| 52 | Droitwich Spa (11) | 2–4 | Coventry United (9) | 58 |
| 53 | Boldmere St. Michaels (9) | 4–1 | GNP Sports (11) | 113 |
| 54 | Heather St. John's (10) | 4–1 | Rocester (10) | 40 |
| 55 | Rugby Town (9) | W.O. | Team Dudley (11) | - |
| Walkover for Rugby Town as Team Dudley were not accepted into the competition. |
| 56 | Racing Club Warwick (10) | 10–0 | Pegasus Juniors (10) | 134 |
| 57 | Melton Town (10) | 2–2 (6–5 p) | Ingles (10) | 128 |
| The FA have insisted the tie to be replayed as it should not have been settled on penalties. |
| 58 | Lutterworth Athletic (10) | 1–3 | Cradley Town (10) | 59 |
| 59 | Wellington (10) | 3–2 | St. Martins (10) | 50 |
| 60 | Dudley Sports (10) | 3–1 | Shawbury United (10) | 48 |
| 61 | Coventry Copsewood (10) | 6–3 | Eccleshall (10) | 25 |
| 63 | Gornal Athletic (11) | 1–2 (a.e.t.) | Stone Old Alleynians (10) | 53 |
| 64 | Wellington Amateurs (11) | W.O. | Ellistown (11) | - |
| Walkover for Wellington Amateurs as Ellistown were not accepted into the competition. |
| 65 | Heath Hayes (10) | 3–1 | Shifnal Town (10) | 44 |
| 66 | Dudley Town (10) | W.O. | Bardon Hill (11) | - |
| Walkover for Dudley Town as Bardon Hill resigned from the competition. |
| 67 | Pershore Town (10) | 0–4 | Hanley Town (9) | 79 |
| 68 | Ashby Ivanhoe (10) | 2–0 | Friar Lane & Epworth (11) | 74 |
| 69 | Bolehall Swifts (11) | 3–0 | Tipton Town (11) | 52 |
| 70 | Haughmond (10) | 2–4 | Saffron Dynamo (11) | 56 |
| 71 | Gedling Miners Welfare (10) | 0–4 | Ilkeston Town (9) | 310 |
| 72 | Rainworth Miners Welfare (10) | 0–3 | Teversal (10) | 64 |
| 73 | Newark Flowserve (10) | W.O. | Harrowby United (10) | - |
| Walkover for Harrowby United as Newark Flowserve were not accepted into the competition. |
| 74 | Leicester Road (10) | 1–1 | Bottesford Town (9) | 35 |
| 75 | Loughborough University (9) | 6–0 | West Bridgford (10) | 78 |
| 76 | Ollerton Town (10) | 1–0 | New Mills (10) | 72 |
| 77 | Skegness Town (10) | 4–1 | Clay Cross Town (11) | 158 |
| 78 | Anstey Nomads (10) | 2–5 | Eastwood Community (10) | 236 |
| 79 | Borrowash Victoria (10) | 0–7 | Hucknall Town (11) | 78 |
| 80 | Clifton All Whites (10) | 4–4 | Belper United (10) | 54 |
| 81 | Blidworth Welfare (11) | 5–3 | Blaby & Whetstone Athletic (11) | 36 |
| 82 | Clipstone (10) | 1–2 | Aylestone Park (10) | 51 |
| 83 | South Normanton Athletic (9) | 3–0 | St. Andrews (10) | 41 |
| 84 | Oadby Town (9) | 1–7 | Sherwood Colliery (10) | 138 |
| 85 | Dunkirk (9) | 3–1 (a.e.t.) | Stapenhill (10) | 46 |
| 86 | Quorn (9) | 5–0 | Retford United (11) | 142 |
| 87 | Holwell Sports (10) | 1–4 | Pinxton (11) | 82 |
| 88 | Lincoln Moorlands Railway (11) | 1–3 | Kimberley Miners Welfare (10) | 52 |
| 89 | Radford (10) | 1–3 | Selston (10) | 52 |
| 90 | Framlingham Town (9) | 3–2 | Wisbech St. Mary (10) | 70 |
| 91 | Deeping Rangers (9) | 7–1 | Blackstones (10) | 109 |
| 92 | Woodbridge Town (9) | 3–1 | Eynesbury Rovers (9) | 154 |
| 93 | Gorleston (9) | 5–1 | Boston Town (9) | 101 |
| 94 | Peterborough Northern Star (9) | 2–0 | Fakenham Town (10) | 30 |
| 96 | Newmarket Town (9) | 2–1 | Downham Town (10) | 67 |
| 97 | Bourne Town (10) | 3–4 | March Town United (10) | 78 |
| 98 | Huntingdon Town (10) | 0–4 | Wroxham (9) | 41 |
| 99 | Ely City (9) | 4–3 (a.e.t.) | Norwich United (9) | 84 |
| 100 | Stotfold (9) | 4–0 | Leyton Athletic (9) | 34 |
| 102 | Newbury Forest (10) | 2–1 | Wivenhoe Town (10) | 30 |
| 104 | Enfield Borough (10) | 6–0 | Halstead Town (10) | 49 |
| 105 | Clapton (9) | 1–1 | Woodford Town (9) | 49 |
| 106 | May & Baker (10) | 8–1 | Burnham Ramblers (10) | 36 |
| 107 | Stanway Rovers (9) | A–A | White Ensign (10) | 50 |
| Match abandoned after 84 minutes due to injury to a White Ensign player when the score was 2–1. |
| 108 | Baldock Town (9) | 2–1 | Long Melford (9) | 66 |
| 109 | Haverhill Rovers (9) | 2–1 | Ilford (9) | 124 |
| 110 | Holland (10) | 4–2 | Cornard United (10) | 45 |
| 111 | Walthamstow (9) | 1–3 (a.e.t.) | Takeley (9) | 85 |
| 112 | Langford (10) | 3–2 | Tower Hamlets (9) | 42 |
| 113 | Southend Manor (9) | 3–0 | Ipswich Wanderers (10) | 40 |
| 114 | Stansted (9) | 1–3 | Brantham Athletic (9) | 48 |
| 115 | London Colney (9) | 2–1 | Cockfosters (9) | 49 |